Describe the expected differences in the impedance for a fully frozen and fully thawed tissue

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Describe the expected differences in the impedance for a fully frozen and fully thawed tissue
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Q.A3
(a)
The biological tissues are complex, anisotropic conductors with resistive and
reactive components. Fig. A3a below shows the distribution of cells in a tissue with
all the relevant components shown including cell membrane (CM), extra-cellular
fluid (RE) and intra-cellular fluid (R1). For such a tissues low-temperature
cryopreservation employing slow-freezing and fast-thawing is considered as a viable
method for the long-term preservation. However, the freezing and thawing processes
themselves can induce injuries to the cells and tissue by damaging the structure and
consequently functionality of the cryopreserved tissue. Bio-impedance
measurements have been proposed as non-invasive method to evaluate the extent of
thawing.
Cell Membrane
Extra-Cellular
Fluid (ECF)
Intra-Cellular
Fluid (ICF)
Fig. A3a: Schematic of cell distribution inside a tissue
Transcribed Image Text:-fleet01-xythos.content.blackboardcdn.com/61b27372e4695/8067545?X-Blackboard-Expiration3D1652702400000&X-Blackbo... Q.A3 (a) The biological tissues are complex, anisotropic conductors with resistive and reactive components. Fig. A3a below shows the distribution of cells in a tissue with all the relevant components shown including cell membrane (CM), extra-cellular fluid (RE) and intra-cellular fluid (R1). For such a tissues low-temperature cryopreservation employing slow-freezing and fast-thawing is considered as a viable method for the long-term preservation. However, the freezing and thawing processes themselves can induce injuries to the cells and tissue by damaging the structure and consequently functionality of the cryopreserved tissue. Bio-impedance measurements have been proposed as non-invasive method to evaluate the extent of thawing. Cell Membrane Extra-Cellular Fluid (ECF) Intra-Cellular Fluid (ICF) Fig. A3a: Schematic of cell distribution inside a tissue
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