Describe the benedict's test. What does the benedict's test detect?
Q: 7. Assuming that these are the 12 microplates. What is wrong with the result of the test? What do…
A: ELISA is an acronym for enzyme-linked immunoassay. Antibodies in the blood are detected using these…
Q: 3. Describe the Fehling's test. What does the Fehling's test detect?
A: Fehling's test is done with the help of Fehling's solution. Fehling's solution consists of copper…
Q: 1. What are the sources, structural descriptions, and biological functions of gelatin, casein, and…
A: Protein is an organic nitrogenous macromolecule that is required for human health. In the human…
Q: 1. What is the purpose of the different reagents used in the procedure of kato-katz technique? 2.…
A: the Kato-Katz approach is used for qualitative and quantitative diagnosis of intestinal helminthic…
Q: advantages and disadvantages of immunofluorenscence test.
A: Immunofluorescence test is a kind of antigen-antibody reaction where a fluorescent dye is tagged to…
Q: Describe the steps in direct sandwich ELISA.
A: ELISAs are a type of immunoassay that are commonly used to quantify levels of a specific target…
Q: What is the purpose of the TSI test?
A: A triple sugar iron test or TSI test is used to test a microorganism's ability to ferment sugar and…
Q: 1: Complete the table below by adding (+) or (–) if each of the sample (amino acid or protein) below…
A: There are different amino acids present in nature. They are primarily involved in protein synthesis…
Q: 1. What is the chemical basis for a positive test in Molisch test? 2. What is the chemical basis for…
A: MOLISCH TEST:- It is a chemical test that is used to detect carbohydrates in a given sample.…
Q: 2. Write the equation involved in all 4 tests above. 1. Osazone test 2. Fehling's test 3. Benedict's…
A: The reactions of carbohydrates are used to identify reducing sugars. Osazone test : This is also…
Q: What is the role of the substrate? Is it really necessary to add substrate in ELISA?Explain why, or…
A: The antibodies that can specifically bind to an antigen can be used for the detection of the…
Q: ) What is the advantage of cryo-sectioning for antibody staining methods? 5) When preparing…
A: Immunohistochemistry can be done in frozen sections or in paraffin-embedded sections. Both frozen…
Q: 4. Put the following steps of the ELISA assay in order (Write the numbers 1-4 in the blank): Add…
A: Introduction: ELISA stands for Enzyme Linked Immunosorbent Assay. It is a commonly used laboratory…
Q: A. What are the analyte, capture molecule, and detector molecule in an ELISA test?
A:
Q: Explain the principle and step by step procedure of Dry Blood Spots collections.?
A: Blood sampling A venous blood sample is usually preferred for routine laboratory tests. Arterial…
Q: Briefl y describe the Kirby-Bauer test and its purpose.
A: Antibiotics are antibacterial drugs. Antibiotic drugs are commonly used in the treatment and…
Q: 1) What are you testing in the picture above? 2) How would you interpret this test? 3) What is the…
A: When Alexander Flemming was working on a bacteria , the culture plate of which was left exposed, he…
Q: 2. Discuss the procedure of Knott's Concentration technique. What are its advantages?
A: Knotts concentration techniques are used for the detection of the microfilariae in the blood. It is…
Q: Explain the pros and cons of 1RM and RM testing. Which test is safer and why?
A: RM i.e. Repetition maximum can be defined as 'maximal number of times a load can be lifted by a…
Q: 4. Draw and give the procedure of harada-mori technique.
A: The Harada-Mori technique is a filter-paper culture technique was performed to increase the chances…
Q: Explain the principles of the Anthony and Maneval's capsule staining procedures?
A: Introduction Generally used at the microscopic level, staining is a technique for enhancing…
Q: 1. What is the chemical basis for a positive test in Barfoed's Test? 2. What is the chemical basis…
A: The qualitative tests are used to detect the presence or absence of a substance. Different…
Q: If you are using sensitivity and specificity to evaluate how well a dichotomous test (like a field…
A: Ideally, when evaluating a dichotomous test like a field sobriety or pregnancy test, we hope to see…
Q: What is the principle behind Liebermann-Burchard test? And What is the clinical significance of…
A: Liebermann-Burchard test used for estimation of unsaturated steroid (cholesterol) and…
Q: 11. Differentiate pUC18 from PBR322 (Give two differences).
A: Plasmid : It is a circular dsDNA molecule . Naturally occurring plasmids are viruses of bacteria.…
Q: 1. What techniques are used in the panel A,B C in figure 1? 2. What does the data show?
A: The provided figure, Figure 1, presents experimental observations related to gene expression errors…
Q: 4) What is the significance of colonies that develop within otherwise clear zones of inhibition? If…
A: Antibiotic susceptibility testing, such as the Kirby-Bauer disk diffusion test, plays a crucial role…
Q: what is ASTO or ASO test? discuss the principle it's important
A: ASTO/ ASO test is Anti- streptolysin O test. Antistreptolysin O is an antibody produced in human…
Q: What is similar about this test and the TSIA test?
A: SIM medium is used to check different parameters such as Sulphur Reduction, Indole Production,…
Q: What is the purpose of Biuret’s test? In biuret’s test, does the color in each test tube containing…
A: There are different biomolecules, including carbohydrates, protein, lipids, etc., are present. The…
Q: 2. What sample shows a positive result with the Molisch Test? Why did this sample give a positive…
A: Note: Please note that we are supposed to give answer only for the first question. so providing…
Q: 1.a)What is the equivalence point and how does it relate to the recommended proportion of serum to…
A: Answer 1) The stoichiometric equivalency between the amount of the reactant being titrated and the…
Q: What is the purpose of a positive control in an ELISA test?
A: The Enzyme-Linked Immunosorbent Assay (ELISA) is a test that detects antibodies or antigens in a…
Q: What is the principle of Bial's test?
A: Bial’s test is known as biochemical test which is used to detect the presence of carbohydrates as…
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- 1. What are the sources, structural descriptions, and biological functions of gelatin, casein, and albumin? 2. Draw the respective chemical reaction involved in Xanthoproteic test, Millon’s test, Sakaguchi test, Hopkin’s-Cole test, and Sulfur test.1. Compare and contrast DAT from IAT based on procedure and clinical significance. 2. Explain the importance of the three phases of Indirect Coomb's test. 3. Differentiate the three potentiators used in Coomb's Test (PEG, LISS, 22% BSA)5. LC50 of drug X acting on the fibroblast cells for 24hrs is going to be determined by MTT assay. a) 5ml of 1 x 10$ cells/ ml cell suspension is required to be prepared for seeding on the cell culture plate for the assay. How that cell suspension is prepared if the following data is given on the cell stock suspension? (6%) Hemocytometer No. of bright dots 1st large square 52 2nd large square 48 3rd large square 51 4th large square Dilution factor: 5 49
- 6. Explain the principle and step by step procedure of Dry Blood Spots collections.?1.What factors are involved in determining the significance of an isolate from a positive blood culture? 2.Is it important to have a full identification on every positive blood culture isolate?3) Were all of the conditions of a standardized Kirby-Bauer test met as you performed this assay? If not, which were not? 4) What is the significance of colonies that develop within otherwise clear zones of inhibition? If the laboratory report for one of your patients indicated colonies within the zone, what concerns would you have for your patient?
- 4) What is the advantage of cryo-sectioning for antibody staining methods? 5) When preparing coverslips with cultured cells for immunofluorescence why is it necessary to coat coverslips with poly-L-lysine or gelatin?REACTIONS OF CARBOHYDRATES Reagents:Samples:10% glucose, galactose, fructose, xylose, maltose, sucrose, lactose, starch (5 ml each) ,2 ml Fehling's A, 2 ml Fehling's B, 8 ml Benedict's reagent, 8 ml Barfoed's reagent, 3 g sodium acetate, 2 g phenylhydrazineHCl Materials: 12( 20 ml) test tubes, test tube rack, test tube brush, test tube holder, alcohol lamp, tripod, wire gauze, water bath, (10 ml) graduated cylinder, dropper, stirring rod, beaker(250 ml), spatula, microscope,7 glass slide, 7 cover slip, watch glass, platform balance Procedure: 1. Osazone formation –Mix 3 g sodium acetate and 2 g phenylhydrazineHCL and 14 ml distilled water. Warm with stirring until solution clears. Place 1 ml sugar solution (glucose, fructose, galactose, maltose, xylose, sucrose, lactose) in separate tube. Add 2 ml of the hot solution in each of the sugar solution, stopper with cotton, mix well and heat in a boiling water bath for 30 minutes. Cool at room temperature and examine the crystals under the…1. What is the purpose of the different reagents used in the procedure of kato-katz technique? 2. How are the Kato Katz Technique results reported? 3. What are the advantages and disadvantages of using Kato-Katz Technique?
