de plant extract. Approximately 19.00 mL of water was added to the plant extract. To determine the protein concentration of the extract, a bovine serum albumin (BSA) stock solution with a concentration of 200 ug mL-1 was used and mixtures with the following compositions and absorbance readings were prepared: A 200 uL aliquot of the diluted plant extract was added with 300 uL water and was subjected to Bradford assay. Determine the protein concentration, in mg mL-1, of the original 1.00 mL crude extract if the absorbance acquired from the analysis is 0.200.
de plant extract. Approximately 19.00 mL of water was added to the plant extract. To determine the protein concentration of the extract, a bovine serum albumin (BSA) stock solution with a concentration of 200 ug mL-1 was used and mixtures with the following compositions and absorbance readings were prepared: A 200 uL aliquot of the diluted plant extract was added with 300 uL water and was subjected to Bradford assay. Determine the protein concentration, in mg mL-1, of the original 1.00 mL crude extract if the absorbance acquired from the analysis is 0.200.
Chemistry for Engineering Students
4th Edition
ISBN:9781337398909
Author:Lawrence S. Brown, Tom Holme
Publisher:Lawrence S. Brown, Tom Holme
Chapter5: Gases
Section: Chapter Questions
Problem 5.107PAE: 107 A soft drink can’s label indicates that the volume of the soda it contains is 12 oz or 355 mL....
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A unique plant was discovered from the hot springs of Maquinit, Coron, Palawan. To study the activity of the plant, approximately 5.00 g of the fresh plant was finely cut and ground and then mixed with an extraction buffer to make up a total of 1.00 mL crude plant extract. Approximately 19.00 mL of water was added to the plant extract. To determine the protein concentration of the extract, a bovine serum albumin (BSA) stock solution with a concentration of 200 ug mL-1 was used and mixtures with the following compositions and absorbance readings were prepared:
A 200 uL aliquot of the diluted plant extract was added with 300 uL water and was subjected to Bradford assay. Determine the protein concentration, in mg mL-1, of the original 1.00 mL crude extract if the absorbance acquired from the analysis is 0.200.
Transcribed Image Text:Tube #
Volume of BSA, mL
Volume of water, mL
Absorbance
1
0.00
2.00
0.000
2
0.40
1.60
0.094
3
0.80
1.20
0.175
14
1.20
0.80
0.282
15
1.60
0.40
0.342
6
2.00
0.00
0.450
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