Answered: de plant extract. Approximately 19.00…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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A unique plant was discovered from the hot springs of Maquinit, Coron, Palawan. To study the activity of the plant, approximately 5.00 g of the fresh plant was finely cut and ground and then mixed with an extraction buffer to make up a total of 1.00 mL crude plant extract. Approximately 19.00 mL of water was added to the plant extract. To determine the protein concentration of the extract, a bovine serum albumin (BSA) stock solution with a concentration of 200 ug mL-1 was used and mixtures with the following compositions and absorbance readings were prepared: A 200 uL aliquot of the diluted plant extract was added with 300 uL water and was subjected to Bradford assay. Determine the protein concentration, in mg mL-1, of the original 1.00 mL crude extract if the absorbance acquired from the analysis is 0.200.

Tube #
Volume of BSA, mL
Volume of water, mL
Absorbance
1
0.00
2.00
0.000
2
0.40
1.60
0.094
3
0.80
1.20
0.175
14
1.20
0.80
0.282
15
1.60
0.40
0.342
6
2.00
0.00
0.450

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