Given the following information calculate the concentration of caffeine for the blank, standards 1-7, and the sample.... ADEGH.K1Important ValuesVolume/ ml(Caffeine)Intensity[Caffeine](ASA)2150ppmCaffeineASABufferSample348ppmBlank2.5volume ASA500ul0.52Total cuvette3000ul20.30.51.76.Bang30.60.51.4ml per can300 mg caffiene per can47310.5Sample81.30.50.79.4fold dilution1.60.50.4107.20.511Sample0.50.612elele

Here Stock concentration of Caffeine given is 150PPM To convert this to µg/ml 1µg/ml = 1PPM 150PPM =…

D. F. G H. Important Values Volume/ml (Caffeine) Intensity 2 Caffeine Buffer (Caffeine] (ASA] 150 ppm ppm ASA Sample 48 Blank 2.5 4 volume ASA 500 ul 0.5 2 Total cuvette 3000 ul 0.3 0.5 1.7 Bang ml per can 300 mg caffiene per can 6 3 0.6 0.5 1.4 473 0.5 Sample 1.3 0.5 0.7 4 fold dilution 1.6 0.5 0.4 10 11 12 2 0.5 Sample 0.5 2 0.6

D. F. G H. Important Values Volume/ml (Caffeine) Intensity 2 Caffeine Buffer (Caffeine] (ASA] 150 ppm ppm ASA Sample 48 Blank 2.5 4 volume ASA 500 ul 0.5 2 Total cuvette 3000 ul 0.3 0.5 1.7 Bang ml per can 300 mg caffiene per can 6 3 0.6 0.5 1.4 473 0.5 Sample 1.3 0.5 0.7 4 fold dilution 1.6 0.5 0.4 10 11 12 2 0.5 Sample 0.5 2 0.6

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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