Consider the following data set: Sample 100 µg/mL CuSO4 standard 200 μg/mL CuSO4 standard 300 μg/mL CuSO4 standard 400 μg/mL CuSO4 standard 500 µg/mL CuSO4 standard CuSO4 Solution with Unknown Concentration Corrected Absorbance at 470 nm 0.032 0.058 0.090 0.114 0.140 0.070 1. Plot (Using MS Excel) or draw by hand a standard calibration curve for samples 1 to 5. Determine the molar absorptivity using the absorbance of the 100 µg/mL CuSO4 standard. (Note: Convert ug/mL to Molarity first using the molar mass of the standard which is 249.685 g/mol). 2. Write the resulting linear regression equation and R2 of the plotted standard calibration curve. 3. Calculate the concentration of the "CuSO4 Solution with Unknown Concentration" using the produced linear regression equation from the standard calibration curve and its absorbance at 470 nm.

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Provide full complete solution do not round off numbers and show proper labelling and cancellations Answer question #3
Consider the following data set:
Sample
100 μg/mL CuSO4 standard
200 μg/mL CuSO4 standard
300 μg/mL CuSO4 standard
400 µg/mL CuSO4 standard
500 μg/mL CuSO4 standard
CuSO4 Solution with Unknown Concentration
Corrected Absorbance at 470 nm
0.032
0.058
0.090
0.114
0.140
0.070
1. Plot (Using MS Excel) or draw by hand a standard calibration curve for samples 1 to 5. Determine the
molar absorptivity using the absorbance of the 100 µg/mL CuSO4 standard. (Note: Convert ug/mL to
Molarity first using the molar mass of the standard which is 249.685 g/mol).
2. Write the resulting linear regression equation and R² of the plotted standard calibration curve.
3. Calculate the concentration of the "CuSO4 Solution with Unknown Concentration" using the produced
linear regression equation from the standard calibration curve and its absorbance at 470 nm.
Transcribed Image Text:Consider the following data set: Sample 100 μg/mL CuSO4 standard 200 μg/mL CuSO4 standard 300 μg/mL CuSO4 standard 400 µg/mL CuSO4 standard 500 μg/mL CuSO4 standard CuSO4 Solution with Unknown Concentration Corrected Absorbance at 470 nm 0.032 0.058 0.090 0.114 0.140 0.070 1. Plot (Using MS Excel) or draw by hand a standard calibration curve for samples 1 to 5. Determine the molar absorptivity using the absorbance of the 100 µg/mL CuSO4 standard. (Note: Convert ug/mL to Molarity first using the molar mass of the standard which is 249.685 g/mol). 2. Write the resulting linear regression equation and R² of the plotted standard calibration curve. 3. Calculate the concentration of the "CuSO4 Solution with Unknown Concentration" using the produced linear regression equation from the standard calibration curve and its absorbance at 470 nm.
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