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Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Consider the endomembrane system. Explain how a CFTR protein would be produced/synthesized in a lung cell
Which organelle of the endomembrane system would have the greatest effect on the production of CFTR if it were to malfunction? Explain your reasoning
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- List and describe the three main types of cytoskeleton. If you wanted to do immunocytochemistry to specifically stain each type of cytoskeleton, what is a protein that could be used for each cytoskeletal type (in other words, what is a unique protein for each cytoskeletal type)? What are three types of actin structures? Describe the involvement of actin structures in cell migration. How is the growth and shrinking of microtubules regulated? Then describe the roles of microtubules in: chromosomal separation during mitosis and the movement of organelles and vesicles within a cell. Describe a possible mechanism on how signaling pathways might impact the cytoskeleton, so that cell migration could be regulated in a localized manner within a multicellular organism. (hint: think about the possible transcriptional regulation of the G-protein regulators) What are 2 main challenges of protein targeting? Then describe one way these challenges are overcome during signal-based targeting and one way…Many eukaryotic organelles are involved in the production and shipping of proteins out of the cell. Explain the pathway that a protein takes from its initial instructions through to its exportation from the cell. Include ALL organelles involved and explain their function.The figure above shows the organization of a protein that will eventually reside in the plasma membrane. The boxes labeled 1 and 2 represent membrane-spanning sequences and the arrow represents a site of action of signal peptidase. Which of the following statements must be TRUE about this protein? O a) Most of this protein will project from the cytosolic side of the membrane. O b) The mature version of this protein will span the membrane twice. c) This is a multi-pass membrane protein. O d) The mature version of this protein will span the membrane once.
- Explain why the statement is correct. Which is true of the ribosomes of endoderm cells of the Cnidaria (jellyfish)? They: are attached to the Rough Endoplasmic reticulum (RER) and carry out the synthesis of the primary structure of proteinsSelect the most accurate explanation for how vesicular transport occurs when a receptor is moving from the plasma memberane to the late endosome/lysosome. A. Endosomes containing the receptor are transported by myosin II walking towards the minus end of microtubules. Endosomes are targeted to the late endosome/lysosome by Rab and SNARES facilitate membrane fusion. B. Endosomes containing the receptor are transported by myosin V walking towards the minus end of microtubules. Endosomes are targeted to the late endosome/lysosome by Rab and SNARES facilitate membrane fusion. OC. Endosomes containing the receptor are transported by dyenin walking towards the minus end of microtubules. Endosomes are targeted to the late endosome/lysosome by Rab and SNARES facilitate membrane fusion. D. Endosomes containing the receptor are transported by kinesin-13 walking towards the plus end of microtubules. Endosomes are targeted to the late endosome/lysosome by clathrin and SNARES facilitate membrane…Stathmin is phosphorylated by cyclin dependent kinases in response to extracellular signals such as growth factors. To further understand the role of phosphorylation in stathmin function, you decide to treat cells with growth factors in the presence or absence of phosphatase and observe microtubule dynamics. Based on the known function of stathmin, and phosphorylation of stathmin, how would you expect phosphatase treatment to affect microtubule dynamics? A. Decreased frequency of catastrophe in the presence of phosphatase, compared to untreated control B. Increased frequency of catastrophe in the presence of phosphatase, compared to untreated control C. Rapid microtubule polymerization in the presence of phosphatase, compared to untreated control D. Catastrophe frequency is the same in phosphatase treated and control cells
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- Discuss the pathway that secretory proteins (proteins destined to leave the cell) take through the endomembrane system starting with their synthesis and ending with their secretion from the cellIN the figure for D: The organization of a protein that normally resides in the plasma membrane in shown in the figure below. The boxes labeled 1,2 3 and 4 represent membrane-spanning sequences and the arrow represents a site of action of signal peptidase. Given this diagram, which of the following statements must be true? (C) (D) oplasmic Reticulum The N-terminus of this protein is in the ER lumen The mature version of this protein will span the membrane twice, with both the N- and C-terminus in the cytoplasm. Two of the answers are correct The C-terminus of this protein is in the ER lumenInsulin is a protein that is produced by pancreatic cells and secreted into the bloodstream. Which of the following options correctly lists the order of the structures in the endomembrane system through which insulin passes from its production to its exit from the cell? rough ER, transport vesicles, Golgi apparatus, vacuole, cell membrane rough ER, Golgi apparatus, smooth ER, cell membrane rough ER, transport vesicles, cell membrane rough ER, lysosomes, transport vesicles, cell membrane