Consider a biochemical reaction that is taking place in a 0.1 M buffer. The initial pH is 7.4, and the pKa of the buffer is 7.2. If, in a final reaction volume of 1.0 mL, 10 micromol of protons are generated, what would be the final pH of the solution?
Consider a biochemical reaction that is taking place in a 0.1 M buffer. The initial pH is 7.4, and the pKa of the buffer is 7.2. If, in a final reaction volume of 1.0 mL, 10 micromol of protons are generated, what would be the final pH of the solution?
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Consider a biochemical reaction that is taking place in a 0.1 M buffer. The initial pH is 7.4, and the pKa of the buffer is 7.2. If, in a final reaction volume of 1.0 mL, 10 micromol of protons are generated, what would be the final pH of the solution?
Expert Solution
Step 1
According to the question we have,
The molarity of buffer in which biochemical reaction takes place = 0.1 M = 0.1 mol/L
The initial pH of the buffer = 7.4
The pKa value of the buffer = 7.2
The volume of proton added = 1.0 ml
The number of moles of protons added = 10 micromol = 10-5 moles ( 1 micro mol = 10-6 moles)
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