Consider a biochemical reaction that is taking place in a 0.1 M buffer. The initial pH is 7.4, and the pKa of the buffer is 7.2. If, in a final reaction volume of 1.0 mL, 10 micromol of protons are generated, what would be the final pH of the solution?

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Consider a biochemical reaction that is taking place in a 0.1 M buffer. The initial pH is 7.4, and the pKa of the buffer is 7.2. If, in a final reaction volume of 1.0 mL, 10 micromol of protons are generated, what would be the final pH of the solution?

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According to the question we have,

The molarity of buffer in which biochemical reaction takes place = 0.1 M = 0.1 mol/L

The initial pH of the buffer = 7.4

The pKa value of the buffer = 7.2

The volume of proton added = 1.0 ml

The number of moles of protons added = 10 micromol = 10-5 moles                   ( 1 micro mol = 10-6 moles)

 

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