You've just started your summer intern as a research assistant in the biology department. Your first assignment is to prepare a pH 7.50 phosphate buffer solution to be used in the isolation of DNA from a cell culture. You've discovered the following items available to you in the lab: 0.514M HPO 4 (K a-4.2x10 13) 48.6 g KOH (molar mass - 56.1g) a. Calculate the pH of your buffer. b. Do you think that this would be a suitable buffer? Explain. SHOW YOUR WORK. Upload your file here.
You've just started your summer intern as a research assistant in the biology department. Your first assignment is to prepare a pH 7.50 phosphate buffer solution to be used in the isolation of DNA from a cell culture. You've discovered the following items available to you in the lab: 0.514M HPO 4 (K a-4.2x10 13) 48.6 g KOH (molar mass - 56.1g) a. Calculate the pH of your buffer. b. Do you think that this would be a suitable buffer? Explain. SHOW YOUR WORK. Upload your file here.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:You've just started your summer intern as a research assistant in the
biology department. Your first assignment is to prepare a pH 7.50 phosphate buffer solution to
be used in the isolation of DNA from a cell culture. You've discovered the following items available to you in the lab:
0.514M HPO 4 (K a-4.2x10 13)
48.6 g KOH (molar mass 56.1g)
a. Calculate the pH of your buffer.
b. Do you think that this would be a suitable buffer? Explain. SHOW YOUR WORK. Upload your file here.
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