Complete the table below regarding the different laboratory tests done for Carbohydrates.Only for the ff:•Lugol’s Test/Iodine Test•Hydrolysis w/Benedict’s Test•Hydrolysis w/Iodine Test Complete the table below regarding the different laboratory tests done for Carbohydrates.TestsChemical Reagents AddedMolisch Testa-naphtholethanolBenedict's TestCu2+ cations (CuSO4.5H2O)0.33M solution of copperBarfoed's Testacetate (Cu(OAc)2)1% acetic acidMoore's TestBial's TestorcinolHydrochloric acid HCIFerric Chloride- resorcinol(m-hydroxybenzene)N Hydrochloric acid HCISeliwanoff's Testglacial acetic acidmixture of phyenylhydrazineOsazone TestHCI and sodium acetateFehling's Test- copper(II) sulphate CuSO4- potassium sodium tartrate(Rochelle salt) C₂H₂O6KNa 4H₂O- sodium hydroxide NaOHTollen's Test-ammoniacal silver nitrateAgH N₂O3Mucic Acid Testconcentrated nitric acidLugol's Test/lodine TestHydrolysis w/Benedict's TestHydrolysis w/lodine TestPositive Results/ Observations(ex: color of solution, precipitate formation, etc)(+) positive result, purple ring formation(+) positive result, yellow-bright orangesolution(+) positive result, formation of red precipitateswithin 3 minutes (monosaccharides) & after 3minutes (disaccharides)(+) positive result, bluish-green color solution(+) positive result, cherry red color solution(+) positive result, formation of crystallinestructure/shape depending on type of reducingsugar(+) positive result, red color solution forglucose and lactose(+) positive result, silver mirror precipitate(+) positive result, formation of crystals at thebottom (presence of galactose or itsderivatives)(+) positive result, red color solution forglucose and lactose(+) positive result, blue colored starch isproduced

There are different types of tests to check the presence of carbohydrates in different chemical…

Complete the table below regarding the different laboratory tests done for Carbohydrates. Only for the ff: •Lugol’s Test/ Iodine Test •Hydrolysis w/ Benedict’s Test •Hydrolysis w/ Iodine Test

Complete the table below regarding the different laboratory tests done for Carbohydrates. Only for the ff: •Lugol’s Test/ Iodine Test •Hydrolysis w/ Benedict’s Test •Hydrolysis w/ Iodine Test

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Complete the table below regarding the different laboratory tests done for Carbohydrates.
Tests
Chemical Reagents Added
Molisch Test
a-naphthol
ethanol
Benedict's Test
Cu2+ cations (CuSO4.5H2O)
0.33M solution of copper
Barfoed's Test
acetate (Cu(OAc)2)
1% acetic acid
Moore's Test
Bial's Test
orcinol
Hydrochloric acid HCI
Ferric Chloride
- resorcinol
(m-hydroxybenzene)
N Hydrochloric acid HCI
Seliwanoff's Test
glacial acetic acid
mixture of phyenylhydrazine
Osazone Test
HCI and sodium acetate
Fehling's Test
- copper(II) sulphate CuSO4
- potassium sodium tartrate
(Rochelle salt) C₂H₂O6KNa 4H₂O
- sodium hydroxide NaOH
Tollen's Test
-ammoniacal silver nitrate
AgH N₂O3
Mucic Acid Test
concentrated nitric acid
Lugol's Test/
lodine Test
Hydrolysis w/
Benedict's Test
Hydrolysis w/
lodine Test
Positive Results/ Observations
(ex: color of solution, precipitate formation, etc)
(+) positive result, purple ring formation
(+) positive result, yellow-bright orange
solution
(+) positive result, formation of red precipitates
within 3 minutes (monosaccharides) & after 3
minutes (disaccharides)
(+) positive result, bluish-green color solution
(+) positive result, cherry red color solution
(+) positive result, formation of crystalline
structure/shape depending on type of reducing
sugar
(+) positive result, red color solution for
glucose and lactose
(+) positive result, silver mirror precipitate
(+) positive result, formation of crystals at the
bottom (presence of galactose or its
derivatives)
(+) positive result, red color solution for
glucose and lactose
(+) positive result, blue colored starch is
produced

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