Complete the calculations and fill in the missing volumes needed below to create a six-tube solute dilution series from a protein stock solution, 0.5 mg/ml BSA (bovine serum albumin), into 1.5 mL microcentrifuge tubes using C1V1 = C2V2, note V2 is not 1.5mL. A table is provided below to record your efforts.
Complete the calculations and fill in the missing volumes needed below to create a six-tube solute dilution series from a protein stock solution, 0.5 mg/ml BSA (bovine serum albumin), into 1.5 mL microcentrifuge tubes using C1V1 = C2V2, note V2 is not 1.5mL. A table is provided below to record your efforts.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Complete the calculations and fill in the missing volumes needed below to create a six-tube solute dilution series from a protein stock solution, 0.5 mg/ml BSA (bovine serum albumin), into 1.5 mL microcentrifuge tubes using C1V1 = C2V2, note V2 is not 1.5mL. A table is provided below to record your efforts.
Transcribed Image Text:4) Complete the calculations and fill in the missing volumes he
from a protein stock solution, 0.5 mg/ml BSA (bovine serum albumin), into 1.5 mL microcentrifuge tubes using CIVI =
C2V2, note V2 is not 1.5mL. A table is provided below to record your efforts.
lution series
New solutions
Volumes
Tube
Concentration
stock solution
DI water
Final volume
1
0.5 mg/mL (stock solution)
250.0 µl
0.0 ul
250.0 ul
2.
0.40 mg/ mL
250.0 µl
0.30 mg/ mL
250.0 ul
4
0.20 mg/ mL
250.0 ul
0.10 mg/ mL
250.0 ul
6.
0.00 mg/ mL
0.0 ul
250.0 ul
250.0 ul
Which tube above can be utilized as your blank for BSA measurements?
3.
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