Compare and contrast the assembly of genomes using Sanger and next-generation sequencing (NGS) approaches

Q: Describe the shotgun method for sequencing an entire genome.

A: Genome or genetic material of a cell is the DNA content present in the nucleus. DNA or…

Q: Need bioinformatics help. 1) Examine the quality of the raw sequencing data 2) Trim low quality…

A: Everything explained in the answer.

Q: Diagram a method for creating a cDNA library

A: cDNA library ( COMPLEMENTARY DNA) It is a combination of cloned cDNA fragments which is inserted…

Q: Describe the components vital for DNA sequencing with the Sanger method

A: DNA sequencing: The process of identifying the correct order of the four nitrogen bases (adenine,…

Q: Write down all possible outcomes for template ATGCCTAAGTTTCCCTAT sequencing

A: Deoxyribonucleic acid (DNA) is the macromolecule that stores genetic information. It is present in…

Q: Identify a problem in molecular biology and how to use CRISPR/cas 9 system to solve it.

A: "CRISPR" is "clustered regularly interspaced short palindromic repeat". It's a piece of DNA that…

Q: (b) Use a drawing to illustrate the principle of DNA gel electrophoresis.

A: Gel electrophoresis is the technique of separation of molecules on the basis of their size and…

Q: Outline the method of RNA sequencing (RNA-Seq).

A: Ribonucleic acid is a type of nucleic acid majorly involved in the gene expression, gene regulation…

Q: Compare the differences in the techniques used in whole genome shotgun sequencing and next…

A: The process of determining the exact order of nucleotides in a gene, a cluster of genes, a…

Q: Discuss FASTA and GenBank file formats and their uses?

A: In the field of bioinformatics and biochemistry, both FASTA and GenBank file formats are important…

Q: Discuss the significance of protein sequencing.

A: Protein sequencing is a process of determining the sequence of amino acids of a protein.

Q: Using the new sequencing platforms as examples discuss how DNA sequencing technologies have evolved…

A: Dna sequencing g is highly essential for the researchers and the scientists to understand different…

Q: list and explain two strategic between the shotgun sequences/ assemble strategy for whole genome…

A: Genomic Sequencing is a process where whole genome of an organism is sequenced. With the help of…

Q: compare and contrast different approaches for assessing genome assemblies using assembly…

A: Genes are hereditary functional units that are made up of DNA (deoxyribonucleic acid). The genome is…

Q: Compare and contrast the use of PCR and gene cloning for amplifying DNA fragments. What are the…

A: Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy of…

Q: tail the process of Sanger sequencing. Include a diagram

A: Sequencing of genetic material involves visualizing the number and arrangement of base pairs. It is…

Q: Compare and contrast bacterial artificial chromosomes (BACs) and yeast artificial chromosomes (YACs)…

A: BACBAC is a cloning vector that is specifically used to propagate DNA fragments in Escherichia coli…

Q: Briefly summarize the steps involved in DNA sequencing anddescribe its applications.

A: DNA sequencing is a technique that is used to determine the correct sequence of DNA by a series of…

Q: Describe two ways in which genome database information is used to study human disease genes.

A: The genome encodes all the information of the deoxyribonucleic acid, the macromolecule containing…

Q: describe sanger sequencing, also known as capillary sequencing, and explain how it works chemically…

A: Dye terminator technique is modified version of the conventional dideoxy chain termination method…

Q: Prepare an X µL PCR reaction mixture and set thermal conditions for gene amplification?

A: PCR is a strong amplification technique that can produce a large amount of a particular DNA segment…

Q: Describe the shotgun method for determining thecomplete genome sequence of an organism.

A: Biotechnology is the branch of science that explain the origin of Genetically modified organisms

Q: Discuss at least three methods of DNA extraction and summarize each methods using a schematic…

A: DNA extraction is a technique to separate DNA from cell membranes, proteins, and other biological…

Q: Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing…

A: The Illumina sequencing chemistry is extremely precise, with the great majority of bases scoring Q30…

Q: Which statement BEST differentiate metagenomics from proteomics? a The material used in…

A: 'Omics' is the suffix added to frequently describe a word that refers to the field of study in…

Q: What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA…

A: Your question contains multiple sub-parts, and as per our policy, we only answer the first three…

Q: Write and briefly define the steps sequentially to clone the gene from the genomic DNA o

A: DNA recombinant molecules from two different types are inserted into living things to produce new…

Q: Describe the process for shotgun sequencing of a genome. Practice aligning the two sets of sequenced…

A: Two questions have been asked. first question has been answered. To get the answer for your second…

Q: Describe the method of a PCR technique in which you can amplify fragments randomly

A: PCR ( Polymerase chain reaction ) is widely used method for synthesis of replica/ clone of DNA . For…

Q: Prepare an 35 µL PCR reaction mixture and set thermal conditions for gene amplification?

A: PCR or the polymerization chain reaction is the molecular biology technique used to make multiple…

Q: Provide five advantages of Next Generation Sequencing? and explain each of these advantages.

A: Here I will discuss about the advantages of next generation sequencing.

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.