coli, mak you believe will grow faster (37° C or 30° C temperature) and why. Results• Time Absorbance 30 °C Absorbance 37 °C Generation time Generation time t=0 min at 30 °C at 37 °C 0.05 0.05 t= 20 min 0.06 0.07 t= 40 min %3D 0.1 0.16 0.28 0.21 0.44 t = 60 min t= 80 min %3D 0.95 Complete the graph. Make sure this figure has a description and all other labels are included. Once you have completed graphing the points, draw a best-fit line for each of your data sets by eye Questions: 1. Which data point can be the first to be considered in log phase for each line? (It will be the first point that seems to be described by your best-fit line.) For the 30° C culture the -minute point is the first one likely to be in log phase. For the 37° C culture the -minute point is the first one likely to be in log phase. Incubation Temperatures - Growth Bio 40- Fall 2019 Page 6

coli, mak you believe will grow faster (37° C or 30° C temperature) and why. Results• Time Absorbance 30 °C Absorbance 37 °C Generation time Generation time t=0 min at 30 °C at 37 °C 0.05 0.05 t= 20 min 0.06 0.07 t= 40 min %3D 0.1 0.16 0.28 0.21 0.44 t = 60 min t= 80 min %3D 0.95 Complete the graph. Make sure this figure has a description and all other labels are included. Once you have completed graphing the points, draw a best-fit line for each of your data sets by eye Questions: 1. Which data point can be the first to be considered in log phase for each line? (It will be the first point that seems to be described by your best-fit line.) For the 30° C culture the -minute point is the first one likely to be in log phase. For the 37° C culture the -minute point is the first one likely to be in log phase. Incubation Temperatures - Growth Bio 40- Fall 2019 Page 6

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Table 3-Determination Tube # Potato Expt. Temp. 2c 3c 4c 5c extract (mL) 2 2 2 2 room temp 20 °C 40 °C 75 °C of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. Boiling 100 °C dH₂O Catechol (mL) (mL) 0 0 0 0 13 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: Absorbance: 25 0.132 Start Time: 4:27 Absorbance: 0.097 2nd Absorbance after 10 min. in Expt Temp. Time for reading: 4:30 Absorbance: 0.128 Time for reading: 4:33 Absorbance: 0.036 0-137 Time for reading: 4:35 Absorbance: Subtract 1st from 2nd absorbance 0.193 Time for reading: 4:37 Absorbance: 0-126 0.023 0.061 0.029 Based on the data from Table 2 answer these questions: Q10) What is the enzyme's optimum temperature? Answer: Q11) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum temperature 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: 0.159 Time for reading: 4:43…