Chlorophylls a and b are plant pigments that absorb sunlight and transfer the energy into photosynthesis of carbohydrates from CO2 and H2O, releasing O2 in the process. Chlorophylls were extracted from chopped up grass and measured by spectrophotometry. The table shows results for chlorophyll a for four separate analysis of five blades of grass. Chlorophyll a (g/L) Blade 1 Blade 2 Blade 3 Blade 4 Blade 5 1.09 1.26 1.1 1.23 0.85 0.86 0.96 1.21 1.3 0.65 0.93 0.8 1.27 0.97 0.86 0.99 0.73 1.12 0.97 1.03 Four replicate measurements for each blade of grass tell us the precision of the analytical procedure (sanalysis). Differences between mean values for each of the five blades of grass are a measure of variation due to sampling (ssampling). Using Excel and it’s ANOVA function, find the standard deviations attributable to sampling and to analysis, as well as the overall standard deviation arising from both sources.

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Chlorophylls a and b are plant pigments that absorb sunlight and transfer the energy into photosynthesis of carbohydrates from CO2 and H2O, releasing O2 in the process. Chlorophylls were extracted from chopped up grass and measured by spectrophotometry. The table shows results for chlorophyll a for four separate analysis of five blades of grass. Chlorophyll a (g/L) Blade 1 Blade 2 Blade 3 Blade 4 Blade 5 1.09 1.26 1.1 1.23 0.85 0.86 0.96 1.21 1.3 0.65 0.93 0.8 1.27 0.97 0.86 0.99 0.73 1.12 0.97 1.03 Four replicate measurements for each blade of grass tell us the precision of the analytical procedure (sanalysis). Differences between mean values for each of the five blades of grass are a measure of variation due to sampling (ssampling). Using Excel and it’s ANOVA function, find the standard deviations attributable to sampling and to analysis, as well as the overall standard deviation arising from both sources.
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Introduction

ANOVA is the measure of the parametric test. It shows three types of variations, they are between variation, within variation, and total variation. It measures the mean significance between the three or more groups. It used the F test to make a conclusion about the data set in anova. 

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