Certain proteins undergo Post Translational Modifications (PTMs) with lipid derivatives such as myristate that are attached to an N-terminal glycine residue of the target protein. Consider the structure of N- myristoyltransferase bound to myristoyl-CoA (PDB.4C2Y). Zoom in on residues 246, 247, 248, and 496 (the C-terminus of the transferase); these residues comprise the active site. Examine how they interact with the cofactor. With this structure in mind, propose a step-wise arrow pushing mechanism for the addition of myristate to an N-terminus of a peptide or protein target. You may use a general acid and/or base as needed.
Certain proteins undergo Post Translational Modifications (PTMs) with lipid derivatives such as myristate that are attached to an N-terminal glycine residue of the target protein. Consider the structure of N- myristoyltransferase bound to myristoyl-CoA (PDB.4C2Y). Zoom in on residues 246, 247, 248, and 496 (the C-terminus of the transferase); these residues comprise the active site. Examine how they interact with the cofactor. With this structure in mind, propose a step-wise arrow pushing mechanism for the addition of myristate to an N-terminus of a peptide or protein target. You may use a general acid and/or base as needed.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Certain proteins undergo Post Translational Modifications (PTMS) with lipid derivatives such as myristate
that are attached to an N-terminal glycine residue of the target protein. Consider the structure of N-
myristoyltransferase bound to myristoyl-CoA (PDB.4C2Y). Zoom in on residues 246, 247, 248, and 496 (the
C-terminus of the transferase); these residues comprise the active site. Examine how they interact with the
cofactor. With this structure in mind, propose a step-wise arrow pushing mechanism for the addition of
myristate to an N-terminus of a peptide or protein target. You may use a general acid and/or base as
needed.
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