Case Study #2
Setting: University Hospital in Santiago de Compostela, Spain.
Subject: 80-year old Caucasian man who developed a pancreatic pseudocyst after presenting with acute necrotizing pancreatitis.
A biopsy of the pseudocyst was obtained following endoscopy using ultrasound fine needle aspiration. The tissue was sent to the hospital lab for analysis. The results follow...
Key to Media Used in Case Studies (shown in picture from left to right)
- Phenol Red Glucose
- Phenol Red Sucrose
- Results holds for lactose too
- Phenol Red Xylose
- SIM
- Read as Sulfur / Indole
- Lysine Decarboxylase
- Ornithine Decarboxylase
- Methyl Red
- Vogues-Proskauer
- Urea
- Simmon’s Citrate
- Nutrient Gelatin
Results of Inoculated Media
Based on the results of the media shown above, determine the causative agent of the pseudocyst. Use the figures in this presentation or in your lab manual for identification.
Test Results:
|
Biochemical test name |
Result for Escherichia coli |
phenol Red Glucose |
Positive |
Phenol Red Sucrose |
Positive |
Phenol Red xylose |
Positive |
SIM |
Positive |
Lysine Decarboxylase |
Negative |
Ornithine decarboxylase |
Negative |
Methyl red
|
Positive |
Vogues-Proskaur |
Negative |
Urea |
Negative |
Simmon's Citrate |
Negative |
Nutrient Gelatine |
Negative |
Step by step
Solved in 2 steps
