Ch 75_case study 3 A 50-yr old male presented with extreme pain in his left thigh. He had an elevated measurement of CPK, an enzyme found predominantly in the heart, brain, and skeletal muscle. When the CPK is elevated, it usually indicates injury or stress to one or more of these areas. His heart muscle CPK fraction was normal. A biopsy specimen was collected by fine-needle aspiration. Gram stain showed GPR with subterminal spores. The aerobic culture was sterile, but the anaerobic culture grew a pure culture of bacteria with the same Gram stain morphology as seen in the direct smear. The colony had irregular edges like a medusa head; a film of growth swarmed over the entire plate by 24 hrs. Questions 1. The isolate was indole-negative. Given this information, what is the genus and species of this bacteria? 2. What does the positive test for CPK indicate in this patient? 3. Infections with this pathogen are an indication of what underlying disease?

Ch 75_case study 3 A 50-yr old male presented with extreme pain in his left thigh. He had an elevated measurement of CPK, an enzyme found predominantly in the heart, brain, and skeletal muscle. When the CPK is elevated, it usually indicates injury or stress to one or more of these areas. His heart muscle CPK fraction was normal. A biopsy specimen was collected by fine-needle aspiration. Gram stain showed GPR with subterminal spores. The aerobic culture was sterile, but the anaerobic culture grew a pure culture of bacteria with the same Gram stain morphology as seen in the direct smear. The colony had irregular edges like a medusa head; a film of growth swarmed over the entire plate by 24 hrs. Questions 1. The isolate was indole-negative. Given this information, what is the genus and species of this bacteria? 2. What does the positive test for CPK indicate in this patient? 3. Infections with this pathogen are an indication of what underlying disease?