Answered: You desperately need to join two DNA…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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You desperately need to join two DNA fragments, one with an EcoRl site and the other with a BamHI site.
Both enzymes leave 5' overhangs cutting after the first base. EcoR/ cuts at gaattc, BamHI at ggatcc. Which
of the following statements is true?

A. You can not ligate these fragments.
B. You can remove the overhangs with another restriction and ligate the blunted ends.
C. You can fill in the ends with DNA polymerase and ligate the blunt ends.
D. This can be done directly as the 5' overhangs anneal.
E. You are better to replace EcoR/ with a related enzyme that leaves a ligatable 3' overhang.

Please explain why it's C

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