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- Why must you first clean debris from items before beginning disinfection or sterilization? (What are the different types of disinfectants? How is each one different or similar from the others? Explain and give examples too.What is the difference between sterilization and disinfection? For lab applications which is preferred?
- What are high level disinfectants? What is an example of one? What is the method of action and the advantages/disadvantages?a) Define 'Disinfection' and 'Sterilization' OR differentiate between them. b) Enlist the different methods of 'Disinfection' which method would you recommend for 'Municipal Water Supply' and WHY? Depending on the availability of 'Source of Water which 'Source' is more important than the other for disinfection and WHY? Give reason. c) of them is stronger than the other and by what percentage? Define or differentiate between “Free Available Chlorine" and “Combined Available Chlorine". Write their Chemical Formulae and mention which one d) Define “Break Point Chlorination". With the help of a neat Figure' show the "Break Point" and also show "Combined Available Chlorine", "Free Available Chlorine" and "Total Available Chlorine". Mention the “Dosage" in order to obtain a “Free Available Chlorine Residual" of about 0.5 mg/l or above. e) Define the term “Chlorine Demand" of water. What are the "Factors" OR enlist the "Factors" that show the "Effectiveness of Chlorine".Why are proteins and amino acids considered to be natural buffers?
- Describe situations that require high-level disinfection and thosefor which medium- or low-level disinfection is adequate.What concentration of ethanol is considered optimum for disinfectant? Why not 100%?What is the difference between a disinfectant and an antiseptic? Which is most effective at removing microbes from a product: sanitization, degerming, or sterilization? Explain
- Define disinfection. Define sterility. Compare and contrast disinfection with sterility.In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperwhat is the difference between sterilizer , disinfectant & surfactant ?