Standard Reduction Potentials of Some Biologically Important Half-ReactionsHalf-reactionE" (V)Pyruvate- + 2H + 2e → lactate--0.185Acetaldehyde + 2H* + 2e- → ethanol-0.197FAD + 2H+ + 2e → FADH2-0.219aGlutathione + 2H* + 2e- → 2 reduced glutathione-0.23S+ 2H* + 2e → H2S-0.243Lipoic acid + 2H* + 2e- → dihydrolipoic acid-0.29NAD* + H* + 2e → NADH-0.320NADP* + H* + 2e- → NADPH-0.324Acetoacetate + 2H* + 2e- > B-hydroxybutyrate-0.346a-Ketoglutarate + CO2 + 2H* + 2e- → isocitrate-0.382H* + 2e- > H2 (at pH 7)--0.414Ferredoxin (Fe3+) + e- → ferredoxin (Fe2+)-0.432Source: Data mostly from R. A. Loach, in Handbook of Biochemistry and Molecular Biology, 3rd edn (G. D. Fasman, ed.), Physicaland Chemical Data, Vol. 1, p. 122, CRC Press, 1976.This is the value for free FAD; FAD bound to a specific flavoprotein (e.g., succinate dehydrogenase) has a different E" that dependson its protein environment. Calculate the standard free-energy change, deltaGo, for the reaction in which acetaldehyde is reduced by the biological electron carrierNADH in the reaction acetaldehyde + NADH + H* → ethanol + NAD*. Then calculate the actual free-energy change, deltaG, when theacetaldehyde is 1.85 M, the NADH is 1.55 M, the ethanol is 0.10 M and the NAD* is 0.10 M, at 28.22°C and pH = 7. Give the actualfree-energy change in kJ/mol to one decimal place. See Table 13-7b for the E° values.Table 13-7b: Standard Reduction Potentials of Some Biologically Important Half-Reactions (link opens in new page)

Standard free energy of biochemical reaction is related to standard reduction potential ∆Go = -nFEo…

Answered: Calculate the standard free-energy…

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter20: Electron Transport And Oxidative Phosphorylation

Section: Chapter Questions

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