Calculate the reagents needed to prepare 10ml of the Basic Common BER Reaction Buffer. Be sure to show all work, including the amount of water necessary Reagent Final Stock Concentration IM pH 7.8 Volume of Stock required Concentration HEPES-KOH pH 25 mM 7.8 KCI 150 mM 2M EDTA MgC12 Glycerol 0.5 M pH 8.0 2M 100% 0.5 mM 2.5 mM 1%

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Chapter1: Chemical Foundations
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2. Calculate the reagents needed to prepare 10ml of the Basic Common BER Reaction Buffer.
Be sure to show all work, including the amount of water necessary
Reagent
Final
Stock
Volume of Stock
Concentration
Concentration
1M pH 7.8
required
HEPES-KOH pH
7.8
25 mM
150 mM
0,5 mM
2 M
0.5 M pH 8.0
2M
KCI
EDTA
MgC12
Glycerol
2,5 mM
1%
100%
Transcribed Image Text:2. Calculate the reagents needed to prepare 10ml of the Basic Common BER Reaction Buffer. Be sure to show all work, including the amount of water necessary Reagent Final Stock Volume of Stock Concentration Concentration 1M pH 7.8 required HEPES-KOH pH 7.8 25 mM 150 mM 0,5 mM 2 M 0.5 M pH 8.0 2M KCI EDTA MgC12 Glycerol 2,5 mM 1% 100%
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