Provide the principle of biuret test used to detect RNA. Explain in 5 sentences essay

Q: ITS DNA was extracted from the same plant but several trials were loaded separately from lanes 2-11.…

A: DNA Extraction --Introduction -- DNA extraction was first performed by Friedrich Miescher in 1869…

Q: limitations of PCR viral diagnostic and explain how it could be improved

A: A significant biochemical technique that is used to generate multiple DNA (deoxyribonucleic acid)…

Q: Provide a detailed description and hand drawn figure of each of the following. (1) Mismatch…

A: Although the genetic variation is important for evolution, the survival of the individual demands…

Q: explain the method of Identifying mutant genes by plasmid librarytransformation

A: Transformation is the horizontal gene transfer by which some bacteria can take up foreign genetic…

Q: A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This…

A: They are  noncellular entities which have their RNA (ribonucleic acid) or DNA(deoxyribonucleic…

Q: What technique could be used to confirm the presence or absence of plasmids in the donor, recipient…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: Define triturate. Define supernatant. Why is SYBR green dye added to the gel? What is the centrifuge…

A: Note:- Please always mention the needed part(s) in case of multiple questions. Thank you! Molecular…

Q: 1 3. 4 Two different cultures of Escherichia coli bacteria were inoculated onto nutrient agar…

A: pGLO plasmid comprises the gene for Green Fluorescent Protein (GFP) and a gene for resistance to the…

Q: At what stage of the culture should bacterial colonies be harvested for plasmid DNA extraction? How…

A: Plasmid training is a way of DNA extraction and purification for plasmid DNA. Many techniques were…

Q: explain  Resistance plasmids

A: Plasmid is a genetic structure in a cell that can replicate independently of the chromosomes,…

Q: What is the purpose of arabinose in the pGLO lab, where is arabinose located, how can it be…

A: Arabinose acts as an allosteric regulator of AraC changing and tells which DNA sites it binds to and…

Q: In the alkaline plasmid screen, what two things separate plasmid from chromosomal DNAs?

A: Following is the procedure for the separation of plasmid by the alkaline lysis method.

Q: Why should plasmid sample be mixed in the inverted position and not vortexed?  Will these actions…

A: Plasmids are small circular pieces of DNA that can be found in many bacteria and are often used in…

Q: Why are there strict reminders about mixing in the inverted position and not vortexing Plasmid…

A: A plasmid is a small, circular, double-stranded DNA molecule that is separate from the chromosomal…

Q: How do we separate plasmid and chromosomal DNA during the alkaline plasmid screen?

A: Alkaline lysis is the most common method used for separating plasmid DNA from chromosomal DNA.

Q: Explain in detail the symbol and abbreviations used in plant protecrion.

A: Plant protection remains an integral part of achieving crop production goals. Plant protection the…

Q: If a concentrated sample is out of the detection range of a chosen dye-based assay, what can be…

A: Dye based assay is a dye binding protein assay based on the binding of protein molecules to…

Q: Discuss in detail, how you would produce recombinant protein subunit vaccines for Influenza virus.

A: Recombinant protein vaccines, which are also known as recombinant subunit vaccines, are designed…

Q: Bacterial mutants can be classified on the basis of the method, Which is those methods?

A: Microorganisms undergo mutations under environmental stress that causes sudden heritable changes in…

Q: Compare and contrast gel-electrophoresis for the separation of nucleic acids and SDS-PAGE for the…

A: In-gel electrophoresis, DNA, RNA, and proteins are separated based on the charge and mass. nucleic…

Q: True or false White colonies in apicillin-agar plates using plasmids with lacZ gene as vector…

A: lacZ is a gene that codes for the enzyme beta-galactosidase, which is involved in lactose metabolism…

Q: We make a 1:5 dilution of our 450 ng/ul PML104 plasmid, and then use 15 of the diluted sample in a…

A: Plasmid digestion refers to the digestion of plasmid using a restriction enzyme. Restriction enzymes…

Q: Describe an electrochemical sensor assay method for rapid bacterial detection and identification.…

A: Electrochemical sensors work on the principle of sensing, recognition and production of electrical…