Provide the principle of biuret test used to detect RNA. Explain in 5 sentences essay
Q: ITS DNA was extracted from the same plant but several trials were loaded separately from lanes 2-11.…
A: DNA Extraction --Introduction -- DNA extraction was first performed by Friedrich Miescher in 1869…
Q: limitations of PCR viral diagnostic and explain how it could be improved
A: A significant biochemical technique that is used to generate multiple DNA (deoxyribonucleic acid)…
Q: Provide a detailed description and hand drawn figure of each of the following. (1) Mismatch…
A: Although the genetic variation is important for evolution, the survival of the individual demands…
Q: explain the method of Identifying mutant genes by plasmid librarytransformation
A: Transformation is the horizontal gene transfer by which some bacteria can take up foreign genetic…
Q: A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This…
A: They are noncellular entities which have their RNA (ribonucleic acid) or DNA(deoxyribonucleic…
Q: Explain the basic principles of Elispot Assay
A: ELISPOT assay is an Enzyme-Linked Immune adsorbent Spot assay that is used for testing cellular…
Q: What technique could be used to confirm the presence or absence of plasmids in the donor, recipient…
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: With detail, compare and contrast the following 5 real-time assays; Taqman, SYBR Green, Molecular…
A: INTRODUCTION It is a real-time polymerase chain reaction also known as qPCR is a Molecular…
Q: Define triturate. Define supernatant. Why is SYBR green dye added to the gel? What is the centrifuge…
A: Note:- Please always mention the needed part(s) in case of multiple questions. Thank you! Molecular…
Q: 1 3. 4 Two different cultures of Escherichia coli bacteria were inoculated onto nutrient agar…
A: pGLO plasmid comprises the gene for Green Fluorescent Protein (GFP) and a gene for resistance to the…
Q: At what stage of the culture should bacterial colonies be harvested for plasmid DNA extraction? How…
A: Plasmid training is a way of DNA extraction and purification for plasmid DNA. Many techniques were…
Q: explain Resistance plasmids
A: Plasmid is a genetic structure in a cell that can replicate independently of the chromosomes,…
Q: What is the purpose of arabinose in the pGLO lab, where is arabinose located, how can it be…
A: Arabinose acts as an allosteric regulator of AraC changing and tells which DNA sites it binds to and…
Q: Explain the main defects of using animal models for viral growth and cultivation.
A: Viruses are highly infectious organisms that cannot be cultivated in non-living culture media. They…
Q: In the alkaline plasmid screen, what two things separate plasmid from chromosomal DNAs?
A: Following is the procedure for the separation of plasmid by the alkaline lysis method.
Q: Give three reasons why Salmonella strains are very useful for mutagenesis studies
A: Ans- The standard set of bacterial strains that are used in the mutagenicity testing of…
Q: How do nucleotide analogs function as therapeutic agents? Explain the mechanism with specific…
A: FUNCTION 1. Nucleotide analogs are nucleotides and which contains a nucleic acid analogs,sugar and a…
Q: Describe ,A Hfr bacterium mates with an F- plasmid containing bacterium. What
A: PLASMIDS- It was discovered that maleness in bacteria is determined by a transmissible genetic…
Q: Why should plasmid sample be mixed in the inverted position and not vortexed? Will these actions…
A: Plasmids are small circular pieces of DNA that can be found in many bacteria and are often used in…
Q: Give typed full explanation Antibiotic sensitivity. What is relationship to ellipse size and…
A: Antibiotic sensitivity testing or antibiotic susceptibility testing is the measurement of the…
Q: Why are there strict reminders about mixing in the inverted position and not vortexing Plasmid…
A: A plasmid is a small, circular, double-stranded DNA molecule that is separate from the chromosomal…
Q: Describe two techniques that can be used to purify viruses and discuss the advantages and…
A: A virus is a microscopic infectious agent that has a simpler structure than cells. Viruses are not…
Q: How do we separate plasmid and chromosomal DNA during the alkaline plasmid screen?
A: Alkaline lysis is the most common method used for separating plasmid DNA from chromosomal DNA.
Q: Explain in detail the symbol and abbreviations used in plant protecrion.
A: Plant protection remains an integral part of achieving crop production goals. Plant protection the…
Q: If a concentrated sample is out of the detection range of a chosen dye-based assay, what can be…
A: Dye based assay is a dye binding protein assay based on the binding of protein molecules to…
Q: Describe the generation of multiple-drug-resistantplasmids
A: Drug inactivation or modification is one mechanism of drug resistance. Changes to the target site.…
Q: Discuss in detail, how you would produce recombinant protein subunit vaccines for Influenza virus.
A: Recombinant protein vaccines, which are also known as recombinant subunit vaccines, are designed…
Q: Bacterial mutants can be classified on the basis of the method, Which is those methods?
A: Microorganisms undergo mutations under environmental stress that causes sudden heritable changes in…
Q: Compare and contrast gel-electrophoresis for the separation of nucleic acids and SDS-PAGE for the…
A: In-gel electrophoresis, DNA, RNA, and proteins are separated based on the charge and mass. nucleic…
Q: True or false White colonies in apicillin-agar plates using plasmids with lacZ gene as vector…
A: lacZ is a gene that codes for the enzyme beta-galactosidase, which is involved in lactose metabolism…
Q: We make a 1:5 dilution of our 450 ng/ul PML104 plasmid, and then use 15 of the diluted sample in a…
A: Plasmid digestion refers to the digestion of plasmid using a restriction enzyme. Restriction enzymes…
Q: explain or describe the principle of immunoturbidimetric C3 Assay.
A: Immunoturbidimetric: A technique that calculates the absorbance of light from an example which is…
Q: During the incubation step of the plaque assay, viral replication leads to host cell lysis. This…
A: The plaque is the clear zone formed by the infection of the virus on the background of the bacterial…
Q: Bacteriophage Lamda Describe what method use to retrieved gene of interest from the plaques.
A: So the lambda phage lacks a contractile tail, it is unable to 'push' its DNA through a bacterial…
Q: Describe an electrochemical sensor assay method for rapid bacterial detection and identification.…
A: Electrochemical sensors work on the principle of sensing, recognition and production of electrical…
Q: How is a specific probe obtained?
A: Probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a…
Q: Write a short discussion based on the result
A: Plasmids are extrachromosomal DNA present in bacteria that are used as vectors for transferring…
Q: use a NanoDrop spectrophotometer to analyze plasmid DNA obtained from miniprep. What information…
A: A spectrophotometer is a device that measures the amount of light a solution has absorbed in order…
Provide the principle of biuret test used to detect RNA. Explain in 5 sentences essay
Step by step
Solved in 2 steps
- Explain in detail the symbol and abbreviations used inplant protecrion.Explain: Describe an electrochemical sensor assay method for rapid bacterial detection and identification. What are the principles and mechanisms involved? functionalization of a sensor array with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase linked DNA oligonucleotide detector probe. Explain how bacteria are being detected using an electrochemical sensorGive three reasons why liposomes/nanoparticles are attractive drug delivery system for recombinant protein preparations?
- AGAROSE GEL ELECTROPHORESIS PRELAB ANSWER ALL QUESTIONS 1. Type of nucleotide is one of the factors that influence electrophoretic mobility. a) True b) False 2. Electrophoresis is used for DNA separation and not proteins. a) True b) False 3. DNA Polymerase is an enzyme to cut DNA into fragments for electrophoresis. a)True b)False 4. Electrophoresis apparatus consists of Gel buffer, chamber and DNA separator. a) True b) False 5. During electrophoresis, DNA fragments collect at the Cathode. a) True b) FalseElectro blotting techniques are routinely used in diagnostic microbiology for detection of a specific DNA sequence in the given bacterial culture. Explain.Explain the basic methods behind the Western blot andcomplement fixation tests.
- what are some advantages to using latex particles instead of blood cells for IM assay?What is the purpose of conducting a modified Furter-Meyer Test? What is the principle behind this test? How could you identify the positive result for this test?The strain of λ phage t is cI857. That tells you that the cI DNA segment is disabled by a specific mutation. What is the exact genetic change in cI857? What specific property of the cI gene product does this mutation change, and how does this help titering for a plaque assay?