Beef catalase has been used to accelerate the decomposition of hydrogen peroxide to yield water and oxygen [Chem. Eng. Educ, 5, 141 (1971)]. The concentration of hydrogen peroxide is given as a function of time for a reaction mixture with a pH of 6.76 maintained at 30°C. I (min) 10 20 50 100 Cr,o, (mol/L) 0.02 0.01775 0.0158 0.0106 0.005 (a) Determine the Michaelis-Menten parameters Vmax and KM. (b) If the total enzyme concentration is tripled, what will the substrate concentration be after 20 minutes?
Beef catalase has been used to accelerate the decomposition of hydrogen peroxide to yield water and oxygen [Chem. Eng. Educ, 5, 141 (1971)]. The concentration of hydrogen peroxide is given as a function of time for a reaction mixture with a pH of 6.76 maintained at 30°C. I (min) 10 20 50 100 Cr,o, (mol/L) 0.02 0.01775 0.0158 0.0106 0.005 (a) Determine the Michaelis-Menten parameters Vmax and KM. (b) If the total enzyme concentration is tripled, what will the substrate concentration be after 20 minutes?
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
Related questions
Question
![Beef catalase has been used to accelerate the decomposition of hydrogen peroxide to yield
water and oxygen [Chem. Eng. Educ, 5, 141 (1971)]. The concentration of hydrogen peroxide is
given as a function of time for a reaction mixture with a pH of 6.76 maintained at 30°C.
I (min)
10
20
50
100
Cro, (mol/L)
0.02
0.01775
0.0158
0.0106
0.005
(a) Determine the Michaelis-Menten parameters Vmax and KM.
(b) If the total enzyme concentration is tripled, what will the substrate concentration be after 20
minutes?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc2a758a4-69fc-4b07-9ca9-f4af1d363e17%2F9e1242a6-740a-4917-822c-154a07d780bf%2Fhlz3pj_processed.png&w=3840&q=75)
Transcribed Image Text:Beef catalase has been used to accelerate the decomposition of hydrogen peroxide to yield
water and oxygen [Chem. Eng. Educ, 5, 141 (1971)]. The concentration of hydrogen peroxide is
given as a function of time for a reaction mixture with a pH of 6.76 maintained at 30°C.
I (min)
10
20
50
100
Cro, (mol/L)
0.02
0.01775
0.0158
0.0106
0.005
(a) Determine the Michaelis-Menten parameters Vmax and KM.
(b) If the total enzyme concentration is tripled, what will the substrate concentration be after 20
minutes?
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 4 steps with 5 images
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemical-engineering and related others by exploring similar questions and additional content below.Recommended textbooks for you
Introduction to Chemical Engineering Thermodynami…
Chemical Engineering
ISBN:
9781259696527
Author:
J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:
McGraw-Hill Education
Elementary Principles of Chemical Processes, Bind…
Chemical Engineering
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
Elements of Chemical Reaction Engineering (5th Ed…
Chemical Engineering
ISBN:
9780133887518
Author:
H. Scott Fogler
Publisher:
Prentice Hall
Introduction to Chemical Engineering Thermodynami…
Chemical Engineering
ISBN:
9781259696527
Author:
J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:
McGraw-Hill Education
Elementary Principles of Chemical Processes, Bind…
Chemical Engineering
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
Elements of Chemical Reaction Engineering (5th Ed…
Chemical Engineering
ISBN:
9780133887518
Author:
H. Scott Fogler
Publisher:
Prentice Hall
Industrial Plastics: Theory and Applications
Chemical Engineering
ISBN:
9781285061238
Author:
Lokensgard, Erik
Publisher:
Delmar Cengage Learning
Unit Operations of Chemical Engineering
Chemical Engineering
ISBN:
9780072848236
Author:
Warren McCabe, Julian C. Smith, Peter Harriott
Publisher:
McGraw-Hill Companies, The