Based on the table below: 1). create a titration curve of pH vs. volume NaOH (graph in in Excel). Indicate on graph the location of each pKa (pka1, pKa2, pKaR) , first and second equivalence point and pI. Show all your calculations. 2). 1st derivative plot of ΔpH / Δvol NaOH an the y-axis and average vol NaOH on the x-axis (graph in Excel). Highlight (label) the equivalence points Table 1.2 - Titration of an unknown amino acid with NaOH Vol. NaOH (mL) pH Avg.Vol. NaOH (mL) pH / Vol NaOH 0.00 2.12 0.5 0.02 1.00 2.14 1.5 -0.02 2.00 2.12 2.5 0.13 3.00 2.25 3.5 0.07 4.00 2.32 4.5 0.06 5.00 2.38 5.5 0.06 6.00 2.44 6.5 0.07 7.00 2.51 7.5 0.1 8.00 2.61 8.5 0.11 9.00 2.72 9.5 0.08 10.00 2.80 10.5 0.29 11.00 3.09 11.5 0.44 12.00 3.53 12.5 1.17 13.00 4.70 13.5 0.51 14.00 5.21 14.5 0.29 15.00 5.50 15.5 0.18 16.00 5.68 16.5 0.16 17.00 5.84 17.5 0.11 18.00 5.95 18.5 0.12 19.00 6.07 19.5 0.11 20.00 6.18 20.5 0.13 21.00 6.31 21.5 0.09 22.00 6.40 22.5 0.14 23.00 6.54 23.5 0.13 24.00 6.67 24.5 0.15 25.00 6.82 25.5 0.18 26.00 7.00 26.5 0.27 27.00 7.27 27.5 0.41 28.00 7.68 28.5 0.49 29.00 8.17 29.5 0.41 30.00 8.58 30.5 0.13 31.00 8.71 31.5 0.17 32.00 8.88 32.5 0.12 33.00 9.00 33.5 0.14 34.00 9.14 34.5 0.13 35.00 9.27 35.5 0.14 36.00 9.41 36.5 0.08 37.00 9.49 37.5 0.11 38.00 9.60 38.5 0.1 39.00 9.70 39.5 0.13 40.00 9.83 40.5 0.12 41.00 9.95 41.5 0.15 42.00 10.10 42.5 0.1 43.00 10.20 43.5 0.37 44.00 10.57 44.5 0.32 45.00 10.89 45.5 0.23 46.00 11.12 46.5 0.2 47.00 11.32 47.5 0.16 48.00 11.48 48.5 0.07 49.00 11.55 49.5 0.1 50.00 11.65 50.5 0.06 51.00 11.71 51.5 0.07 52.00 11.78 52.5 0.04 53.00 11.82 53.5 0.04 54.00 11.86 54.5 0.05 55.00 11.91 55.5 0.04 56.00 11.95 56.5 0.02 57.00 11.97 57.5 0.02 58.00 11.99 58.5 0.01 59.00 12.00 29.5 0.20339 - At what pH does your amino acid carry a net charge of 0? Draw the structure Identify the unknown from the following four :
Based on the table below:
1). create a titration curve of pH vs. volume NaOH (graph in in Excel). Indicate on graph the location of each pKa (pka1, pKa2, pKaR) , first and second equivalence point and pI. Show all your calculations.
2). 1st derivative plot of ΔpH / Δvol NaOH an the y-axis and average vol NaOH on the x-axis (graph in Excel). Highlight (label) the equivalence points
Table 1.2 - Titration of an unknown amino acid with NaOH
|
Vol. NaOH (mL) |
pH |
Avg.Vol. NaOH (mL) |
pH / Vol NaOH |
|
0.00 |
2.12 |
0.5 |
0.02 |
|
1.00 |
2.14 |
1.5 |
-0.02 |
|
2.00 |
2.12 |
2.5 |
0.13 |
|
3.00 |
2.25 |
3.5 |
0.07 |
|
4.00 |
2.32 |
4.5 |
0.06 |
|
5.00 |
2.38 |
5.5 |
0.06 |
|
6.00 |
2.44 |
6.5 |
0.07 |
|
7.00 |
2.51 |
7.5 |
0.1 |
|
8.00 |
2.61 |
8.5 |
0.11 |
|
9.00 |
2.72 |
9.5 |
0.08 |
|
10.00 |
2.80 |
10.5 |
0.29 |
|
11.00 |
3.09 |
11.5 |
0.44 |
|
12.00 |
3.53 |
12.5 |
1.17 |
|
13.00 |
4.70 |
13.5 |
0.51 |
|
14.00 |
5.21 |
14.5 |
0.29 |
|
15.00 |
5.50 |
15.5 |
0.18 |
|
16.00 |
5.68 |
16.5 |
0.16 |
|
17.00 |
5.84 |
17.5 |
0.11 |
|
18.00 |
5.95 |
18.5 |
0.12 |
|
19.00 |
6.07 |
19.5 |
0.11 |
|
20.00 |
6.18 |
20.5 |
0.13 |
|
21.00 |
6.31 |
21.5 |
0.09 |
|
22.00 |
6.40 |
22.5 |
0.14 |
|
23.00 |
6.54 |
23.5 |
0.13 |
|
24.00 |
6.67 |
24.5 |
0.15 |
|
25.00 |
6.82 |
25.5 |
0.18 |
|
26.00 |
7.00 |
26.5 |
0.27 |
|
27.00 |
7.27 |
27.5 |
0.41 |
|
28.00 |
7.68 |
28.5 |
0.49 |
|
29.00 |
8.17 |
29.5 |
0.41 |
|
30.00 |
8.58 |
30.5 |
0.13 |
|
31.00 |
8.71 |
31.5 |
0.17 |
|
32.00 |
8.88 |
32.5 |
0.12 |
|
33.00 |
9.00 |
33.5 |
0.14 |
|
34.00 |
9.14 |
34.5 |
0.13 |
|
35.00 |
9.27 |
35.5 |
0.14 |
|
36.00 |
9.41 |
36.5 |
0.08 |
|
37.00 |
9.49 |
37.5 |
0.11 |
|
38.00 |
9.60 |
38.5 |
0.1 |
|
39.00 |
9.70 |
39.5 |
0.13 |
|
40.00 |
9.83 |
40.5 |
0.12 |
|
41.00 |
9.95 |
41.5 |
0.15 |
|
42.00 |
10.10 |
42.5 |
0.1 |
|
43.00 |
10.20 |
43.5 |
0.37 |
|
44.00 |
10.57 |
44.5 |
0.32 |
|
45.00 |
10.89 |
45.5 |
0.23 |
|
46.00 |
11.12 |
46.5 |
0.2 |
|
47.00 |
11.32 |
47.5 |
0.16 |
|
48.00 |
11.48 |
48.5 |
0.07 |
|
49.00 |
11.55 |
49.5 |
0.1 |
|
50.00 |
11.65 |
50.5 |
0.06 |
|
51.00 |
11.71 |
51.5 |
0.07 |
|
52.00 |
11.78 |
52.5 |
0.04 |
|
53.00 |
11.82 |
53.5 |
0.04 |
|
54.00 |
11.86 |
54.5 |
0.05 |
|
55.00 |
11.91 |
55.5 |
0.04 |
|
56.00 |
11.95 |
56.5 |
0.02 |
|
57.00 |
11.97 |
57.5 |
0.02 |
|
58.00 |
11.99 |
58.5 |
0.01 |
|
59.00 |
12.00 |
29.5 |
0.20339 |
- At what pH does your amino acid carry a net charge of 0? Draw the structure
Identify the unknown from the following four :
1. The titration curve of an unknown amino acid is needed to be plot in the given problem. The pH of the solution of the amino acid after the addition of the different volume of the NaOH solution is given.
Trending now
This is a popular solution!
Step by step
Solved in 7 steps with 6 images
