Based on the resulting chromatograms after visualization with iodine vapor, which between the solvent systems WHITE and MIX resulted in a more effective separation of the pigments of the indigo extract? Explain your answer in terms of the solvent that did not effectively separate the components of the root extract.
Based on the resulting chromatograms after visualization with iodine vapor, which between the solvent systems WHITE and MIX resulted in a more effective separation of the pigments of the indigo extract? Explain your answer in terms of the solvent that did not effectively separate the components of the root extract.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Based on the resulting chromatograms after visualization with iodine vapor, which between the solvent systems WHITE and MIX resulted in a more effective separation of the pigments of the indigo extract? Explain your answer in terms of the solvent that did not effectively separate the components of the root extract.
Transcribed Image Text:A. THIN LAYER CHROMATOGRAPHY
Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubia
tinctorum, commonly known as rose madder. Earth spotted the root extract on two
hydrophobic silica gel plates. He then placed each plate in separate developing chambers,
one containing solvent system WHITE and the other housing solvent system MIX. Earth
allowed the chromatograms to develop for around five minutes. Afterwards, he removed
the plates from the containers and placed them inside an iodine chamber. The
chromatograms resulting from the TLC experiment after visualization are illustrated as
follows. The distances travelled by each spot and by the solvents were measured and
recorded in the displayed table.
D
75.0 mm
71.5 mm
DISTANCE
SOLVENT
SPOTS
TRAVELLED FROM
SYSTEM
solvent
THE ORIGIN, mm
front
A
13.0
В
32.5
WHITE
41.0
D
66.5
A
10.5
B
27.5
MIX
A
- origin,
A
39.0
D
53.5
10.5 mm
8 mm
WHITE
MIX
O mm-
O mm
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