b) What glassware and lab equipment would you need?

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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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b) What glassware and lab equipment would you need?
Transcribed Image Text:b) What glassware and lab equipment would you need?
1. This question is adapted from Harris 19-D.
Carnosine is a dipeptide whose antioxidant properties protect cells from free radicals. Carnosine was
determined by derivatization with naphthalene-2,3-dicarboxyaldehyde and cyanide followed by
fluorescence detection using excitation at 445 nm and emission at 490. Quantification was by standard
addition. To four aliquots of 20-ul cell lysate were added volumes of 100µM carnosine standard to
generate final concentrations of 0, 1.0, 2.5, and 5.0 µM added carnosine. Solutions were diluted to 70µl
before addition of 15µl of 5 mM naphthalene-2,3-dicarboxyaldehyde and 15µl of 10 mM NaCN.
H
H2N.
OH
Carnosine
HN.
Naphthalene-2,3-
dicarboxyaldehyde
Cyanide
OH
Fluorescent product
HN.
Fluorescence intensity
Concentration (uM) of added carnosine
in final 100 µL
0.0
0.465
1.0
0.698
2.5
1.029
5.0
1.651
Transcribed Image Text:1. This question is adapted from Harris 19-D. Carnosine is a dipeptide whose antioxidant properties protect cells from free radicals. Carnosine was determined by derivatization with naphthalene-2,3-dicarboxyaldehyde and cyanide followed by fluorescence detection using excitation at 445 nm and emission at 490. Quantification was by standard addition. To four aliquots of 20-ul cell lysate were added volumes of 100µM carnosine standard to generate final concentrations of 0, 1.0, 2.5, and 5.0 µM added carnosine. Solutions were diluted to 70µl before addition of 15µl of 5 mM naphthalene-2,3-dicarboxyaldehyde and 15µl of 10 mM NaCN. H H2N. OH Carnosine HN. Naphthalene-2,3- dicarboxyaldehyde Cyanide OH Fluorescent product HN. Fluorescence intensity Concentration (uM) of added carnosine in final 100 µL 0.0 0.465 1.0 0.698 2.5 1.029 5.0 1.651
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