B с A Tube pH 4.5 Buffer (μl) water ul 250 250 250 250 250 1 2 3 4 5 6 7 250 250 500 450 400 350 300 250 225 D E G 150mM Sucrose (extract (μl) DNSA μl Absorbance 250 800 250 250 250 250 250 250 0 50 100 150 200 250 275 800 800 800 800 800 800 H Reducing sugar μmoles Sucrose added (umoles) Starting sucrose concentration [S] (mm) 0 7.5 15 22.5 30 37.5 41.25
B с A Tube pH 4.5 Buffer (μl) water ul 250 250 250 250 250 1 2 3 4 5 6 7 250 250 500 450 400 350 300 250 225 D E G 150mM Sucrose (extract (μl) DNSA μl Absorbance 250 800 250 250 250 250 250 250 0 50 100 150 200 250 275 800 800 800 800 800 800 H Reducing sugar μmoles Sucrose added (umoles) Starting sucrose concentration [S] (mm) 0 7.5 15 22.5 30 37.5 41.25
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question
I need help how to find value for starting sucrose concentration column J
the reducing sugar I will find by comparing Absorbance to standard curve from previous experiment no need to worry bout G and H column
Thank you
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A
Tube
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B
pH 4.5 Buffer (μl) water ul
250
250
250
250
250
250
250
500
450
400
350
300
250
225
D
E
150m M Sucrose (extract (μl)
250
250
250
0
50
100
150
200
250
275
250
250
250
250
F
G
DNSA μl Absorbance
800
800
800
800
800
800
800
H
Reducing sugar μmoles Sucrose added (µmoles) Starting sucrose concentration [S] (mm)
0
7.5
15
22.5
30
37.5
41.25](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc418f0cd-165a-4ea5-9a24-6d9fb76e817b%2F9a1bfd9d-44fa-4b50-a6b1-ef8e4283e635%2Fan6e6vw_processed.jpeg&w=3840&q=75)
Transcribed Image Text:1
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7
8
9
10
11
17
A
Tube
1
2
3
4
5
6
7
с
B
pH 4.5 Buffer (μl) water ul
250
250
250
250
250
250
250
500
450
400
350
300
250
225
D
E
150m M Sucrose (extract (μl)
250
250
250
0
50
100
150
200
250
275
250
250
250
250
F
G
DNSA μl Absorbance
800
800
800
800
800
800
800
H
Reducing sugar μmoles Sucrose added (µmoles) Starting sucrose concentration [S] (mm)
0
7.5
15
22.5
30
37.5
41.25
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