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At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as defined below: erase sucrose (designated as A) products Starting with a sucrose concentration CAo = 1.0 millimol/liter and an enzyme concentration Cg, = 0.01 millimole/liter, the following kinetic data are obtained in a batch reactor (concentrations are calculated from optical rotation measurements): Ca, millimole/liter 0.84 0.68| 0.53| 0.38 | 0.27 | 0.16| 0.09| 0.04 || 0.01 | 0.00 || 0.002 t,hr 3 4 7 8 10 11 Determine whether these data can be reasonably fitted by the kinetic equation of the Michaelis-Menten type or k,C,Ce -TA = Ca + CM where C, = Michaelis Constant CA and ra If the fit is reasonable, determine kg and CM-

At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as defined below: erase sucrose (designated as A) products Starting with a sucrose concentration CAo = 1.0 millimol/liter and an enzyme concentration Cg, = 0.01 millimole/liter, the following kinetic data are obtained in a batch reactor (concentrations are calculated from optical rotation measurements): Ca, millimole/liter 0.84 0.68| 0.53| 0.38 | 0.27 | 0.16| 0.09| 0.04 || 0.01 | 0.00 || 0.002 t,hr 3 4 7 8 10 11 Determine whether these data can be reasonably fitted by the kinetic equation of the Michaelis-Menten type or k,C,Ce -TA = Ca + CM where C, = Michaelis Constant CA and ra If the fit is reasonable, determine kg and CM-

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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Linearization Problem 1 At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as defined below: SACrase sucrose (designated as A) → products Starting with a sucrose concentration CAo = 1.0 millimol/liter and an enzyme concentration Cg, = 0.01 millimole/liter, the following kinetic data are obtained in a batch reactor (concentrations are calculated from optical rotation measurements): Ca, millimole/liter 0.84 0.68 0.53 0.38 | 0.27 0.16 0.09 0.04 0,00 0.002 0.01 8 9 5 11 t,hr 1 2 3 4 7 10 Determine whether these data can be reasonably fitted by the kinetic equation of the Michaelis-Menten type or k3C,Czo Ca + CM -ra = where Cy = Michaelis Constant CA and r = If the fit is reasonable, determine kg and CM-
Transcribed Image Text:Linearization Problem 1 At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as defined below: SACrase sucrose (designated as A) → products Starting with a sucrose concentration CAo = 1.0 millimol/liter and an enzyme concentration Cg, = 0.01 millimole/liter, the following kinetic data are obtained in a batch reactor (concentrations are calculated from optical rotation measurements): Ca, millimole/liter 0.84 0.68 0.53 0.38 | 0.27 0.16 0.09 0.04 0,00 0.002 0.01 8 9 5 11 t,hr 1 2 3 4 7 10 Determine whether these data can be reasonably fitted by the kinetic equation of the Michaelis-Menten type or k3C,Czo Ca + CM -ra = where Cy = Michaelis Constant CA and r = If the fit is reasonable, determine kg and CM-
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