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At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as defined below: merase sucrose (designated as A) → products Starting with a sucrose concentration Cao = 1.0 millimol/liter and an enzyme concentration C, = 0.01 millimole/liter, the following kinetic data are obtained in a batch reactor (concentrations are calculated from optical rotation measurements): Ca, millimole/liter 0.84 | 0.68 | 0.53| 0.38 0.27 0.16 0.09 0.04 | 0.01 0.00 0.002 8 9 t, hr 1 3 4 7 8 10 11 Determine whether these data can be reasonably fitted by the kinetic equation of the Michaelis-Menten type or k3C,Czo Ca + CM where C = Michaelis Constant CA and ra If the fit is reasonable determine k- and C.

At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as defined below: merase sucrose (designated as A) → products Starting with a sucrose concentration Cao = 1.0 millimol/liter and an enzyme concentration C, = 0.01 millimole/liter, the following kinetic data are obtained in a batch reactor (concentrations are calculated from optical rotation measurements): Ca, millimole/liter 0.84 | 0.68 | 0.53| 0.38 0.27 0.16 0.09 0.04 | 0.01 0.00 0.002 8 9 t, hr 1 3 4 7 8 10 11 Determine whether these data can be reasonably fitted by the kinetic equation of the Michaelis-Menten type or k3C,Czo Ca + CM where C = Michaelis Constant CA and ra If the fit is reasonable determine k- and C.

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
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Linearization Problem 1 At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as defined below: sucrose (designated as A) merase products Starting with a sucrose concentration C4o = 1.0 millimol/liter and an enzyme concentration Cg, = 0.01 millimole/liter, the following kinetic data are obtained in a batch reactor (concentrations are calculated from optical rotation measurements): C4, millimole/liter 0.84 0.68 | 0.53| 0.38 | 0.27 | 0.16| 0.09| 0.04 | 0.01 | 0.00 0.002 8 6 5 t,hr 1 2 3 4 6 7 8 9 10 11 Determine whether these data can be reasonably fitted by the kinetic equation of the Michaelis-Menten type or k3C,Ceo Ca + Cxe where C = Michaelis Constant CA and ra = If the fit is reasonable, determine kg and C-
Transcribed Image Text:Linearization Problem 1 At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as defined below: sucrose (designated as A) merase products Starting with a sucrose concentration C4o = 1.0 millimol/liter and an enzyme concentration Cg, = 0.01 millimole/liter, the following kinetic data are obtained in a batch reactor (concentrations are calculated from optical rotation measurements): C4, millimole/liter 0.84 0.68 | 0.53| 0.38 | 0.27 | 0.16| 0.09| 0.04 | 0.01 | 0.00 0.002 8 6 5 t,hr 1 2 3 4 6 7 8 9 10 11 Determine whether these data can be reasonably fitted by the kinetic equation of the Michaelis-Menten type or k3C,Ceo Ca + Cxe where C = Michaelis Constant CA and ra = If the fit is reasonable, determine kg and C-
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