Asparagine and glutamine residues in proteins are deamidated in an aqueous solution to yield aspartate and glutamate, respectively, and ammonium.  Proteins undergoing deamidation of one or more of their Asn or Gln groups have been detected by isoelectric focusing.  Why would isoelectric focusing be effective in separating deamidated from non-deamidated protein?  Compare the relative pI values of the deamidated and non-deamidated proteins.

Q: TABLE 2: EFFECT OF TEMPERATURE ON CATALASE ACTIVITY STRENGTH OF REACTION Temperature…

A: The explanation is given below-

Q: From a study of protein A as a function of temperature, we find that the ratio of unfolded protein…

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Q: A graph showing the difference between wavelength and absorptions 1,6 1,4 1,2 0,8 - Arginine 0,6 -…

A: When there is a wavelength where the absorption is strongest an absorption spectrum is at maximum…

Q: The electrospray ionization mass spectrum of the tripeptide Ala-Met-Thr shows peaks of…

A: Given peptide is: Ala-Met-Thr

Q: † 14.) A reversible inhibitor that is unable to bind to the ES complex A) always binds in the active…

A: que . 1 A reversible inhibitor that is unable to bind to the ES complex  --B) may be an…

Q: How many potential binding sites do you see in chymotrypsin? What is the chemical nature of each…

A: Chymotrypsin is an enzyme that acts in the digestive system of the organisms. It acts as a catalyst…

Q: The isoelectric point, pl, of the protein horse liver alcohol dehydrogenase is 6.8, while that of…

A: In the provided information and questions, we deal with the concept of isoelectric points (pI) and…

Q: dP AvapH dT T(V, – Vi) AvapH _ AvapH TV, AvapH -P RT2 %3D %3D RT T AvapH For a change of state,…

A: The equation given is,

Q: 4b) Canavanine is closely related to arginine, and like arginine its side group has a +1 charge when…

A: Canavanine has a similar structure of Arginine.  Hence Canavanine has 3 groups that can be…

Q: 8. The following proteins represent a wide range of molecular weights and isoelectric points. Mr is…

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Q: The amino acid cysteine has three ionizable groups with the indicated pKas values. Draw the shapes…

A: Given is amino acid .The name of amino acid is cysteine. It has three ionisable groups.

Q: Protease enzymes cleave proteins by hydrolyzing peptide bonds. The strategy for each type of…

A: Proteases, also known as peptidases or proteinases, play a major role in the digestion and…

Q: Please create the titration curve of aspartate and annotate the corresponding isoforms.

A: Amino acids are amphoteric in nature due to the presence their ionizable α-amino and α-carboxylic…

Q: Estimate the pI of a dipeptide Cys-Asp assuming that the pKa values for αα-NH3+, αα-COOH, side chain…

A: The question is based on the concept of peptides. we have to identify the isoelectric point of given…

Q: ted elution order of lysine, aspartic acid, and phenylalanine? ning

A: The order of elution depends on the side chains of the amino acids. The side chains will either lose…

Q: Which of the folowing saturation curves show a ligand that most strongly binds to the protein? 1.0-…

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Q: Review Topic [References) The isoelectric point, pl, of the protein lipase A is 4.9 , while that of…

A: At isoelectric point is the point where the net charge on protein becomes zero.

Q: One of the beolow statements is FALSE Amytase Pepain Arginese 4. 10 12 O a. Irreversible enzyme…

A: The given graph in the question is a plot of activity of an enzyme vs. pH of the medium. In this…

Q: 2) Part of the 'H NMR spectrum for a dipeptide recorded in D₂O is shown below. The signal for the CH…

A: When molecules are placed in a strong magnetic field, the nuclei of some atoms will begin to behave…

Q: Calculate isoelectric point (PI) of the peptide GSTSRASPRM. pKa N-terminus = 9.3; pKa R = 12.5; pKa…

A: The peptide is GSTSRASPRM.

Q: 4. What are the various modes of coordination of the imidazole ring in histidine and the carboxylate…

A: The coordination of amino acid residues to metal ions is a critical component of metalloproteins'…

Q: How would I calculate the isoelectric point for a peptide (LSGHKKRDDQ) that has zwitter ion after…

A: To calculate the isoelectric point (pI) for a peptide, you can use the Henderson-Hasselbalch…

Q: Based on the experimental results shown in the table, Identify whether the inhibitor acting on this…

A: "Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: X Incorrect. For 10PF, how would the removal of residues 114-131 affect solute selectivity, assuming…

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Q: cch the hyd of the helic

A: The amino acids can be classified based on the polarity of the side chains to form a scale called…

Q: 5 (a) Describe in detail how you will determine the primary structure of protein. (c) You have been…

A: Amino acids are those hydrocarbons that have a central carbon atom which is attached to a carboxylic…

Q: If the pH of a solution of Arginine is equal to a half of the 3 pka values of all of its functional…

A: Half of the three pKa values indicates that the sum of the three pKa values divided by 2.

Q: Please consider the structural formula of the amino acid shown below when answering the following…

A: (A) draw the form of this amino acid that predominates at pH 8  This pH is highly basic so that both…

Q: For an enzyme-catalyzed reaction, the presence of 10 nM of a reversible inhibitor yields a Vmax…

A: Answer is given below

Q: 8. Identify the inhibitor from the following list for the selective target of the enzyme having C in…

A: o Acrylamide groups act as Michael acceptors and thus react with nucleophiles like thiol (SH) groups…

Q: Show where trypsin would cleave the following peptide. Identify the cleavage sites by dragging the…

A: Here we are required to show the cut of the peptide trypsin

Q: Complete the following reaction: Naz-peptide -Peptide (A) CH والی CH3 2P3 NA₂ CH H NH₂ V=0

A: SNAr reactionHydrolysis

Q: Two phenylalanine residues are separated by 1.0 nm in a polypeptide. Calculate the dispersion…

A: Dispersion force is the force of attraction that exist between atoms or molecules that developed…

Q: Indicate the d-electron count for the iron surfur protein given below. A) 2 B) 6 C) 8 D) 4 E) 10

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Q: Kinetics data were gathered for an enzyme-catalyzed reaction under identical conditions both with…

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Q: 5. An enzyme catalyzes the reaction M = concentration of 1 nM, and the Vmax is 2 µM s-1. The Km for…

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Q: For the determination of Pb in blood, a 5.00 mL sample was treated with trichloroacetic acid to…

A: The given data is as follows.      A1 = 0.396 (first point)      A2 = 0.599 (second point)      C1 =…

Q: Which of the following protein assays does not make use of the copper (I or II) ions in the…

A: The purpose of the protein assay is to determine the concentration of a protein out of different…

Q: When the relaxivity of a Gd-based magnetic resonance agent is high, this means that slight changes…

A: The given statement is False. Slight changes in Gd concentration will result into big changes in…

Q: What is the value of KspKsp for Tl2SO4?

A: Given , Ti2SO4

Q: Draw the relevant chemical structures for each protonation state of Glutamate (Glu) and calculate…

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.