Approximately how much does staphylococcal nuclease (Table 11-1) decrease the etivationenergy of its reaction at 25 C (hydrolysis of phosphodiester bond)?

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Approximately how much does staphylococcal nuclease (Table 11-1) decrease the
activationenergy of its reaction at 25 C (hydrolysis of phosphodiester bond)?
TABLE 11-1 Catalytic Power of Some Enzymes
Nonenzymatic
Reaction Rate (s~l)
Enzymatic Reaction
Rate (s-)
Enzyme
Rate Enhancement
Carbonic anhydrase
1.3 x 10-!
1x 10
Chorismate mutase
7.7 X 10
2.6 x 10-5
50
1.9 x 10
Triose phosphate isomerase
4.3 x 10-6
4300
1.0 x 10°
Carboxypeptidase A
3.0 x 10
578
1.9 x 10"
AMP nucleosidase
1.0 x 10-11
60
6.0 × 1012
Staphylococcal nuclease
1.7 x 10-13
Source: Radzicka, A. and Wolfenden, R., Science 267, 91 (1995).
95
5.6 x 1014
Transcribed Image Text:Approximately how much does staphylococcal nuclease (Table 11-1) decrease the activationenergy of its reaction at 25 C (hydrolysis of phosphodiester bond)? TABLE 11-1 Catalytic Power of Some Enzymes Nonenzymatic Reaction Rate (s~l) Enzymatic Reaction Rate (s-) Enzyme Rate Enhancement Carbonic anhydrase 1.3 x 10-! 1x 10 Chorismate mutase 7.7 X 10 2.6 x 10-5 50 1.9 x 10 Triose phosphate isomerase 4.3 x 10-6 4300 1.0 x 10° Carboxypeptidase A 3.0 x 10 578 1.9 x 10" AMP nucleosidase 1.0 x 10-11 60 6.0 × 1012 Staphylococcal nuclease 1.7 x 10-13 Source: Radzicka, A. and Wolfenden, R., Science 267, 91 (1995). 95 5.6 x 1014
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