Antibodies that bind to specific proteins are import­ant tools for defining the locations of molecules in cells.The sensitivity of the primary antibody—the antibody thatreacts with the target molecule—is often enhanced byusing labeled secondary antibodies that bind to it. Whatare the advantages and disadvantages of using secondaryantibodies that carry fluorescent tags versus those thatcarry bound enzymes?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Antibodies that bind to specific proteins are import­
ant tools for defining the locations of molecules in cells.
The sensitivity of the primary antibody—the antibody that
reacts with the target molecule—is often enhanced by
using labeled secondary antibodies that bind to it. What
are the advantages and disadvantages of using secondary
antibodies that carry fluorescent tags versus those that
carry bound enzymes?

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