An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments is tabulated below. Glycogen (mM) Product Formed Glycogen only (mM/min) Product Formed Glycogen and Inhibitor (mM/min) 0.5 22.6 15.9 1.0 32.1 24.9 1.5 38.1 28.2 2.0 41.2 35.1 2.5 44.7 40.0 3.0 48.5 43.3 Draw the Michaelis-Menten and Lineweaver-Burke plots of these dat In the attachment. Determine the form of inhibition observed from these results, and explain your rationale for this form. Determine the values for Km and Vmax from these results
An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments is tabulated below. Glycogen (mM) Product Formed Glycogen only (mM/min) Product Formed Glycogen and Inhibitor (mM/min) 0.5 22.6 15.9 1.0 32.1 24.9 1.5 38.1 28.2 2.0 41.2 35.1 2.5 44.7 40.0 3.0 48.5 43.3 Draw the Michaelis-Menten and Lineweaver-Burke plots of these dat In the attachment. Determine the form of inhibition observed from these results, and explain your rationale for this form. Determine the values for Km and Vmax from these results
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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- An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments is tabulated below.
Glycogen (mM) |
Product Formed Glycogen only (mM/min) |
Product Formed Glycogen and Inhibitor (mM/min) |
0.5 |
22.6 |
15.9 |
1.0 |
32.1 |
24.9 |
1.5 |
38.1 |
28.2 |
2.0 |
41.2 |
35.1 |
2.5 |
44.7 |
40.0 |
3.0 |
48.5 |
43.3 |
- Draw the Michaelis-Menten and Lineweaver-Burke plots of these dat
In the attachment.
- Determine the form of inhibition observed from these results, and explain your rationale for this form.
- Determine the values for Km and Vmax from these results.
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