An enzyme-catalyzed reaction has a Km of 1 mM and a Vmax of 4 nmole/L sec-1. The reaction velocity (nmole/L sec-1) when the substrate concentration is 0.25 mM is: A. 1.25 B.  10.0 C.  5.0 D. 0.50 E. 1.0 F. 100 G. 150 H. 55 I. 75

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  1. An enzyme-catalyzed reaction has a Km of 1 mM and a Vmax of 4 nmole/L sec-1. The reaction velocity (nmole/L sec-1) when the substrate concentration is 0.25 mM is:

A. 1.25

B.  10.0

C.  5.0

D. 0.50

E. 1.0

F. 100

G. 150

H. 55

I. 75

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