1. For enzymatic reaction, a mechanism was proposed by Michaelis and Menten as follows: E + S == ES k, and k,' ES → E and P k2. Use steady state assumption, derive expression for the reaction rate. Where E is concentration of enzyme, S substrate, ES complex of E and S, E = E, – ES. (If you have difficulty in doing it, please consult lecture note)

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1. For enzymatic reaction, a mechanism was proposed by Michaelis and Menten as follows:
E + S == ES k, and k,'
ES → E and P k2.
a. Use steady state assumption, derive expression for the reaction rate. Where E is concentration
of enzyme, S substrate, ES complex of E and S, E = E, – ES. (If you have difficulty in doing it,
please consult lecture note)
b. Assume Km = 0.038 mol.L1 at 25 °C, when the substrate concentration is 0.156 Mol.L1, the rate
of the reaction is 1.21 m mol/L.s. The maximum rate of conversion reaction is reached at high
substrate concentrations. Calculate the maximum rate of this enzyme catalyzed reaction.
Transcribed Image Text:1. For enzymatic reaction, a mechanism was proposed by Michaelis and Menten as follows: E + S == ES k, and k,' ES → E and P k2. a. Use steady state assumption, derive expression for the reaction rate. Where E is concentration of enzyme, S substrate, ES complex of E and S, E = E, – ES. (If you have difficulty in doing it, please consult lecture note) b. Assume Km = 0.038 mol.L1 at 25 °C, when the substrate concentration is 0.156 Mol.L1, the rate of the reaction is 1.21 m mol/L.s. The maximum rate of conversion reaction is reached at high substrate concentrations. Calculate the maximum rate of this enzyme catalyzed reaction.
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