Ammonia (NH3) is a weak base that under acidic conditions becomes protonated to the ammonium ion in thefollowing reaction:NH3 + H+ → NH4 +NH3 freely permeates biological membranes, including thoseof lysosomes. The lysosome is a subcellular organelle witha pH of about 4.5–5.0; the pH of cytoplasm is about 7.0.What is the effect on the pH of the fluid content of lysosomes when cells are exposed to ammonia? Note: Ammonium (NH4 +) does not diffuse freely across membranes.

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Ammonia (NH3) is a weak base that under acidic conditions becomes protonated to the ammonium ion in the
following reaction:
NH3 + H+ → NH4 +
NH3 freely permeates biological membranes, including those
of lysosomes. The lysosome is a subcellular organelle with
a pH of about 4.5–5.0; the pH of cytoplasm is about 7.0.
What is the effect on the pH of the fluid content of lysosomes when cells are exposed to ammonia? Note: Ammonium (NH4 +) does not diffuse freely across membranes.

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