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ALBUMIN CONCEN- BIURET REAGENT TOTAL STOCK (10 MG/ML) TUBE WATER TRATION ABSORBANCE (A) VOLUME (MG/ML) 1. Blank 5 ml. O mL 3 ml 2. Std. 1 4 mL 1ml 3 ml 0.1 3. Std. 2 3 ml 2 ml 3 ml 0.2 4. Std. 3 2 mL 3 ml 3 ml 0.3 5. Std. 4 1 ml. 4 mL 3 ml 0.4 6. Std. 5 O mL 5 ml 3 mL 1.5 5 ml of unk. + 3 mL of Biuret reagent Absorbance of unk. 7. Unk. Tube = 0.38 A

ALBUMIN CONCEN- BIURET REAGENT TOTAL STOCK (10 MG/ML) TUBE WATER TRATION ABSORBANCE (A) VOLUME (MG/ML) 1. Blank 5 ml. O mL 3 ml 2. Std. 1 4 mL 1ml 3 ml 0.1 3. Std. 2 3 ml 2 ml 3 ml 0.2 4. Std. 3 2 mL 3 ml 3 ml 0.3 5. Std. 4 1 ml. 4 mL 3 ml 0.4 6. Std. 5 O mL 5 ml 3 mL 1.5 5 ml of unk. + 3 mL of Biuret reagent Absorbance of unk. 7. Unk. Tube = 0.38 A

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Using the data table below- calculate the concentration of the albumin in each of the test tubes labeled 1 to 6,determine the total volume in each of the test tubes. Prepare a well labeled best fit line graph using the absorbance given, and using the given absorbance of the unknown sample 7 determine the unknown protein concentration. Submit the graph of the data to your instructor electronically following the instructions provided by your instructor.

### Biuret Assay for Protein Concentration Determination #### Table of Contents: 1. **Introduction** 2. **Materials and Methods** 3. **Results and Analysis** 4. **Conclusion** --- ### 1. Introduction The Biuret Assay is a chemical test that allows the detection of proteins in a sample. The absorbance of the final solution is measured spectrophotometrically, which is proportional to the protein concentration. ### 2. Materials and Methods For this assay, a series of standard solutions and an unknown sample are prepared with varying concentrations of albumin stock solution. The following table displays the detailed preparation of these solutions, along with their respective absorbance readings: | Tube | Water | Albumin Stock (10 mg/mL) | Biuret Reagent | Concentration (mg/mL) | Total Volume | Absorbance (A) | |------------|-------|--------------------------|----------------|-----------------------|--------------|----------------| | 1. Blank | 5 mL | 0 mL | 3 mL | 0 | | 0 | | 2. Std. 1 | 4 mL | 1 mL | 3 mL | | | 0.1 | | 3. Std. 2 | 3 mL | 2 mL | 3 mL | | | 0.2 | | 4. Std. 3 | 2 mL | 3 mL | 3 mL | | | 0.3 | | 5. Std. 4 | 1 mL | 4 mL | 3 mL | | | 0.4 | | 6. Std. 5 | 0 mL | 5 mL | 3 mL | | | 0.5 | | 7. Unk. | | | | | | | | | | | 5 mL of unk. + | 3 mL of Biuret reagent| | | | | | | | | | 0.38 | **Notes:** - For all tubes,
Transcribed Image Text:### Biuret Assay for Protein Concentration Determination #### Table of Contents: 1. **Introduction** 2. **Materials and Methods** 3. **Results and Analysis** 4. **Conclusion** --- ### 1. Introduction The Biuret Assay is a chemical test that allows the detection of proteins in a sample. The absorbance of the final solution is measured spectrophotometrically, which is proportional to the protein concentration. ### 2. Materials and Methods For this assay, a series of standard solutions and an unknown sample are prepared with varying concentrations of albumin stock solution. The following table displays the detailed preparation of these solutions, along with their respective absorbance readings: | Tube | Water | Albumin Stock (10 mg/mL) | Biuret Reagent | Concentration (mg/mL) | Total Volume | Absorbance (A) | |------------|-------|--------------------------|----------------|-----------------------|--------------|----------------| | 1. Blank | 5 mL | 0 mL | 3 mL | 0 | | 0 | | 2. Std. 1 | 4 mL | 1 mL | 3 mL | | | 0.1 | | 3. Std. 2 | 3 mL | 2 mL | 3 mL | | | 0.2 | | 4. Std. 3 | 2 mL | 3 mL | 3 mL | | | 0.3 | | 5. Std. 4 | 1 mL | 4 mL | 3 mL | | | 0.4 | | 6. Std. 5 | 0 mL | 5 mL | 3 mL | | | 0.5 | | 7. Unk. | | | | | | | | | | | 5 mL of unk. + | 3 mL of Biuret reagent| | | | | | | | | | 0.38 | **Notes:** - For all tubes,
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