After gel electrophoresis, it is common to extract a protein (or proteins) from a band in the gel, digest the proteins with a protease such as chymotrypsin, and sequence the resulting peptide fragments. If a protein from a gel band is extracted, subjected to digestion with chymotrypsin, and then run on another gel under same conditions as the first gel BUT FOUND TO MIGRATE TO THE SAME POSITION on the gel, the most likely explanation is that (Assume that a control protein sample digested with the chymotrypsin enzyme gives the expected cleavage pattern.) no proteolysis occurred - something is wrong with the chymotrypsin active site. something is likely wrong with the 2nd gel. no proteolysis occurred - the protein doesn't contain any F, T, or W amino acids. no proteolysis occurred - the protein doesn't contain any K or R residues.
After gel electrophoresis, it is common to extract a protein (or proteins) from a band in the gel, digest the proteins with a protease such as chymotrypsin, and sequence the resulting peptide fragments. If a protein from a gel band is extracted, subjected to digestion with chymotrypsin, and then run on another gel under same conditions as the first gel BUT FOUND TO MIGRATE TO THE SAME POSITION on the gel, the most likely explanation is that (Assume that a control protein sample digested with the chymotrypsin enzyme gives the expected cleavage pattern.) no proteolysis occurred - something is wrong with the chymotrypsin active site. something is likely wrong with the 2nd gel. no proteolysis occurred - the protein doesn't contain any F, T, or W amino acids. no proteolysis occurred - the protein doesn't contain any K or R residues.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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