After determining the optimum pH, you are now tasked to determine the inhibitory effects of acarbose, a known anti-diabetic drug which acts an inhibitor of alpha-glucosidase, an intestinal enzyme that hydrolyzes starch to release glucose for absorption and energy usage of the body. You studied the rates of the enzyme at different glycogen concentrations with and without the presence of 0.10 mM acarbose. Below is the summary for this procedure. a) What is the Vmax, in arbitrary units, of the uninhibited reaction (without acarbose)? b) What is the KM , in mM, of the uninhibited reaction (without acarbose)? c) What is the type of inhibition?
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
After determining the optimum pH, you are now tasked to determine the inhibitory effects of acarbose, a known anti-diabetic drug which acts an inhibitor of alpha-glucosidase, an intestinal enzyme that hydrolyzes starch to release glucose for absorption and energy usage of the body. You studied the rates of the enzyme at different glycogen concentrations with and without the presence of 0.10 mM acarbose. Below is the summary for this procedure.
a) What is the Vmax, in arbitrary units, of the uninhibited reaction (without acarbose)?
b) What is the KM , in mM, of the uninhibited reaction (without acarbose)?
c) What is the type of inhibition?
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