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Absorbance 0.9 0.6 0.3 L ד T 0 0.8 0.4 blank Abs. 20 40 Cnitrite nitrite 7.1 μM A=0.0209C+0.0608 R² = 0.9999 A=0.0115C+0.0249 R² = 0.9993 A=0.00407C+0.0011 R² = 0.9991 21.4 μM 14.3 μM 28.6 μΜ 35.7 μM 30 Time, min P = 50 mm P = 30 mm P = 10 mm

Absorbance 0.9 0.6 0.3 L ד T 0 0.8 0.4 blank Abs. 20 40 Cnitrite nitrite 7.1 μM A=0.0209C+0.0608 R² = 0.9999 A=0.0115C+0.0249 R² = 0.9993 A=0.00407C+0.0011 R² = 0.9991 21.4 μM 14.3 μM 28.6 μΜ 35.7 μM 30 Time, min P = 50 mm P = 30 mm P = 10 mm

Chemistry & Chemical Reactivity
Chemistry & Chemical Reactivity
9th Edition
ISBN:9781133949640
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions
Section4.8: Stoichiometry Of Reactions In Aqueous Solution-titrations
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In an effort to reduce costs and increase the accessibility of instruments that utilize spectrophotometric detection, some researchers are beginning to experiment with 3D-printed parts. One example of this is the 3D-printed flow cell, shown at right. This device was made using polylactic acid and accommodates a LED at one end and a detector at the other. It can be used for standalone flow injection spectrophotometry or coupled to a chromatographic separation to be used as a detector. 

    1. Explain why the sensitivity varies with the length of the flow cell, as shown in the data below.
    2. Could this setup be used for fluorescence analysis? Why or why not?
Absorbance 0.9 0.6 0.3 L ד T 0 0.8 0.4 blank Abs. 20 40 Cnitrite nitrite 7.1 μM A=0.0209C+0.0608 R² = 0.9999 A=0.0115C+0.0249 R² = 0.9993 A=0.00407C+0.0011 R² = 0.9991 21.4 μM 14.3 μM 28.6 μΜ 35.7 μM 30 Time, min P = 50 mm P = 30 mm P = 10 mm
Transcribed Image Text:Absorbance 0.9 0.6 0.3 L ד T 0 0.8 0.4 blank Abs. 20 40 Cnitrite nitrite 7.1 μM A=0.0209C+0.0608 R² = 0.9999 A=0.0115C+0.0249 R² = 0.9993 A=0.00407C+0.0011 R² = 0.9991 21.4 μM 14.3 μM 28.6 μΜ 35.7 μM 30 Time, min P = 50 mm P = 30 mm P = 10 mm
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