A newly established sub-national testing and referral center for hematologic diseases is conductingscreening procedure to establish the reference values for mothers and their newborns who are bothpresumed to be normal or healthy. As part of the screening procedure, hemoglobin levels and hemoglobinfractionation and quantification using high perfommance liquid chromatography (HPLC) were perfommed onthe mother and her newborn infant. The mother's hemoglobin concentration was 125 g/L, and thenewborn's was 210 g/L. The mother's hemoglobin fractions were quantified as 97% Hb A, 2% Hb A2, and1% Hb F by HPLC. The newborn's results showed 88% Hb F, 10% Hb A, and 2% Hb A2.1. a.Were these hemoglobin results within expected reference intervals?b. Why were the mother's and the newborn's hemoglobin concentration so different?What is the difference between the test to determine the hemoglobin concentration and the test to analyzehemoglobin by HPLC?с.

Hemoglobin: Hemoglobin is protein present in red blood cells, the main function of hemoglobulin is…

Answered: a. Were these hemoglobin results within…

Phlebotomy Essentials

6th Edition

ISBN:9781451194524

Author:Ruth McCall, Cathee M. Tankersley MT(ASCP)

Publisher:Ruth McCall, Cathee M. Tankersley MT(ASCP)

Chapter1: Phlebotomy: Past And Present And The Healthcare Setting

Section: Chapter Questions

Problem 1SRQ

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1. If the MCHC value is decreased, what term is used to describe the peripheral blood smear finding? 2. Aside from automated and microhematocrit determinations, what red blood cell index can be used for indirect measurement of hematocrit? 3. Automated and microhematocrit determinations present discrepancy in the results due to what factor? 4. What is the wavelength used in the hemiglobincyanide method of hemoglobin determination? (Answer in this format: numerical value space abbreviated unit)
What would the order of elution be (first to last to come out of a size-exclusion chromatography column) for the mixture of myoglobin (17.7 kDa), hemoglobin (64.5 kDa), lysozyme (14.3 kDa) & albumin (67 kDa)? a. Albumin, hemoglobin, lysozyme, myoglobin Ob. Hemoglobin, myoglobin, lysozyme, albumin Oc. Lysozyme, myoglobin, albumin, hemoglobin Od. Albumin, hemoglobin, myoglobin, lysozyme
A 35 year old woman of Chinese ethnic background (Guangdong province) presented with a near normal Haemoglobin level of 112 g/L. On performing a Full Blood Count (FBC) she was found to have an erythrocyte Mean Cell Volume (MCV) of 64.9fL and a Mean Cell Haemoglobin (MCH) value of 20.6 pg.A family study was performed on her father, mother and younger brother, including capillary electrophoresis. The following values were found:Father Mother Brother Patient Normal Range Age 74 69 32 35Hb (g/L) 98 107 113 112 133-167 (m) 118-148 (f)MCV (fL) 69 89.4 62.7 64.9 80-100MCH (pg) 22.5 29.2 20.1 20.6 28-32HbA (%) 98.0 97.2 97.8 98.0 97-98HbA2 (%) 2.0 2.7 2.2 2.0 1.5-3RBC (x1012/L)…
What is the diagnostic importance of examining blood smears? Bone marrow smear?
Compute for the absolute count of patients A and B PATIENT A WBC count = 6 x 109/L Differential count Absolute count Neutrophils = 65% Lymphocytes = 20% Monocytes = 13% Eosinophils = 2% PATIENT B WBC count = 18 x 109/L Differential count Absolute count Neutrophils = 65% Lymphocytes = 20% Monocytes = 10% Eosinophils = 5% Give an impression of their results. (Indicate whether the cells are increased or decreased using the proper terminologies. Indicate also whether the increased or decreased values are absolute or relative)
Which test measures the average size of red blood cells?
Analyse the graph depicting changes in precision value along slices showing the segmentation of the right atrium from a CT scan. Find any trends regarding the precision value of the segmentation of the right atrium from the medical image CT scan
which of the following can you conclude about the hemoglobin in individuals with sickle cell anemia? Choose two answers that apply.
Refer to the flow cytometry data below: b. 1.10 Bote PSCA a. Is this data represented as a "dot plot" or histogram? b. What does Forward Scatter (FSC) represent? c. What does Side Scatter (SSC) represent? d. What is the purpose of "gating"? Identify the gate(s) in the data above.
Give typed explanation RR is a 22 y/o female admitted for suspected GI bleeding. She is 5’1’’ and 92lbs. Calculate her CrCl if her serum creatinine is 0.7mg/dL.
Calculate the average number of RBC’s per cubic millimeter of the following blood sample on the given hemocytometer.
Explain the diagnostic importance of blood testing?

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a. Were these hemoglobin results within expected reference intervals? b. Why were the mother's and the newborn's hemoglobin concentration so different? c. What is the difference between the test to determine the hemoglobin concentration and the test to analyze hemoglobin by HPLC?