A. Preparation of Nutrient Broth a. To 3 grams of beef extract (0.3%) and( 5 grams of peptone (0.5%) add distilled water to make 1 liter broth. ( 1liter of water + Knorr beef cubes) b. Hasten the solution of these materials by warming. c. Weight out the amount of peptone needed and immediately close the bottle tightly to prevent absorption of moisture from the atmosphere. Beef extract, a hydrolyzed extract of meat at a concentration of 0.3 – 0.5% will support the growth of microorganism when combined with peptone. It contains peptides and amino acids, nucleotide fractions, organic acids, minerals and some vitamins. Its function is to complete the properties of peptone by contributing minerals, phosphates, energy sources and those essential factors missing in peptone. Peptones are hydrolyzed products of protein brought about by the action of the enzyme's protease. Peptones are commonly used as nitrogen source but may also supply other requirements such as Na+, Cl-, P04= and trace metals. Peptones used in carbohydrate fermentation should not contain fermentable carbohydrates. Meat peptones may contain traces of glycogen but normally free in highly utilizable carbon compound such as lactate. Yeast extracts and yeast peptones are considered to be substantial sources of water-soluble vitamins in culture media.

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.