a) Which restriction enzyme should you use to cleave the plasmid? Explain your answer.

Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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1. You wish to insert a gene in the plasmid below. (Note the neon green lines
represent restriction sites.)
amp gene
BamHI
Sacl
Sacl
EcoRI
BamHI
33
BamHI
Gene of interest
Chromosomal DNA
from human cells
a) Which restriction enzyme should you use to cleave the plasmid? Explain
your answer.
Transcribed Image Text:1. You wish to insert a gene in the plasmid below. (Note the neon green lines represent restriction sites.) amp gene BamHI Sacl Sacl EcoRI BamHI 33 BamHI Gene of interest Chromosomal DNA from human cells a) Which restriction enzyme should you use to cleave the plasmid? Explain your answer.
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