a) Which restriction enzyme should you use to cleave the plasmid? Explain your answer.

Biology: The Dynamic Science (MindTap Course List)
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Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
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Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications
Section: Chapter Questions
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1. You wish to insert a gene in the plasmid below. (Note the neon green lines
represent restriction sites.)
amp gene
BamHI
Sacl
Sacl
EcoRI
BamHI
33
BamHI
Gene of interest
Chromosomal DNA
from human cells
a) Which restriction enzyme should you use to cleave the plasmid? Explain
your answer.
Transcribed Image Text:1. You wish to insert a gene in the plasmid below. (Note the neon green lines represent restriction sites.) amp gene BamHI Sacl Sacl EcoRI BamHI 33 BamHI Gene of interest Chromosomal DNA from human cells a) Which restriction enzyme should you use to cleave the plasmid? Explain your answer.
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