A student has been asked to prepare a reaction mixture, which is suitable to test the activity of an enzyme in dependence of the pH of the solution. The mixture contains a buffer compound, which will facilitate a stable pH. Now they have to determine the ratio of conjugate base/weak acid required such that they will obtain the desired pH. They have been provided with the buffer parameters provided in the image below. Determine the ratio with two decimal places precision. B_A ratio_007 Note: You may need to round the numbers to get the required answer. A/ Final pH of solution: 5.8 Final volume of solution: 100 ml Buffer compound concentration: 150 mM Buffer compound pk₂: 6.3

A student has been asked to prepare a reaction mixture, which is suitable to test the activity of an enzyme in dependence of the pH of the solution. The mixture contains a buffer compound, which will facilitate a stable pH. Now they have to determine the ratio of conjugate base/weak acid required such that they will obtain the desired pH. They have been provided with the buffer parameters provided in the image below. Determine the ratio with two decimal places precision. B_A ratio_007 Note: You may need to round the numbers to get the required answer. A/ Final pH of solution: 5.8 Final volume of solution: 100 ml Buffer compound concentration: 150 mM Buffer compound pk₂: 6.3

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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