A student has been asked to prepare a reaction mixture, which is suitable to test the activity of an enzyme in dependence of the pH of the solution. The mixture contains a buffer compound, which will facilitate a stable pH. Now they have to determine the ratio of conjugate base/weak acid required such that they will obtain the desired pH. They have been provided with the buffer parameters provided in the image below. Determine the ratio with two decimal places precision. B_A ratio_007 Note: You may need to round the numbers to get the required answer. A/ Final pH of solution: 5.8 Final volume of solution: 100 ml Buffer compound concentration: 150 mM Buffer compound pk₂: 6.3
A student has been asked to prepare a reaction mixture, which is suitable to test the activity of an enzyme in dependence of the pH of the solution. The mixture contains a buffer compound, which will facilitate a stable pH. Now they have to determine the ratio of conjugate base/weak acid required such that they will obtain the desired pH. They have been provided with the buffer parameters provided in the image below. Determine the ratio with two decimal places precision. B_A ratio_007 Note: You may need to round the numbers to get the required answer. A/ Final pH of solution: 5.8 Final volume of solution: 100 ml Buffer compound concentration: 150 mM Buffer compound pk₂: 6.3
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:A student has been asked to prepare a reaction mixture, which is suitable to test the activity of an enzyme in dependence of
the pH of the solution.
The mixture contains a buffer compound, which will facilitate a stable pH. Now they have to determine the ratio of conjugate
base/weak acid required such that they will obtain the desired pH. They have been provided with the buffer parameters
provided in the image below.
Determine the ratio with two decimal places precision.
B_A ratio_007
Note: You may need to round the numbers to get the required answer.
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Final pH of solution:
5.8
Final volume of solution:
100 ml
Buffer compound
concentration:
150 mM
Buffer compound pk₂:
6.3
Page 4 of 12
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