A student completes their TLC experiment, but can not visualize any spots on their plate under a UV lamp. What advise would NOT be very helpful to this student?
A student completes their TLC experiment, but can not visualize any spots on their plate under a UV lamp. What advise would NOT be very helpful to this student?
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Make sure your compound actually absorbs UV light. You can check your spots under a lamp before running the TLC. |
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Make sure your spot line is not getting submerged with solvent. If the spots are submerged, they will get dissolved before the TLC has run. |
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Try to spot more sample on the spots using more repititions of spotting or a more concentrated spotting solution. |
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Try to use a more polar eluting solvent. |
Given:
A student completes their TLC experiment, but can not visualize any spots on their plate under a UV lamp.
To Determine: The advise that would NOT be very helpful to this student.
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