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A scientist wants to separate a mixture of two proteins. Protein A has a pl of 3.2 and protein B has a pl of 10.5 making them ideal candidates for separation by ion exchange chromatography. The scientist chooses to run the experiment at neutral pH using a positively charged resin. Which of the following statements about this experiment are true? i. Protein A will elute out of the column before protein B. ii. Both of the proteins, A and B, will bind to the resin. iii. A higher concentration of salt solution would be needed to elute protein A from the column than to elute protein B. iv. We would expect to see equally good separation if the scientist had chosen to use a pH of 2

A scientist wants to separate a mixture of two proteins. Protein A has a pl of 3.2 and protein B has a pl of 10.5 making them ideal candidates for separation by ion exchange chromatography. The scientist chooses to run the experiment at neutral pH using a positively charged resin. Which of the following statements about this experiment are true? i. Protein A will elute out of the column before protein B. ii. Both of the proteins, A and B, will bind to the resin. iii. A higher concentration of salt solution would be needed to elute protein A from the column than to elute protein B. iv. We would expect to see equally good separation if the scientist had chosen to use a pH of 2

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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A scientist wants to separate a mixture of two proteins. Protein A has a pl of 3.2 and protein B has a pl of 10.5 making them ideal candidates for separation by ion exchange chromatography. The scientist chooses to run the experiment at neutral pH using a positively charged resin. Which of the following statements about this experiment are true? i. Protein A will elute out of the column before protein B. ii. Both of the proteins, A and B, will bind to the resin. iii. A higher concentration of salt solution would be needed to elute protein A from the column than to elute protein B. iv. We would expect to see equally good separation if the scientist had chosen to use a pH of 2
Transcribed Image Text:A scientist wants to separate a mixture of two proteins. Protein A has a pl of 3.2 and protein B has a pl of 10.5 making them ideal candidates for separation by ion exchange chromatography. The scientist chooses to run the experiment at neutral pH using a positively charged resin. Which of the following statements about this experiment are true? i. Protein A will elute out of the column before protein B. ii. Both of the proteins, A and B, will bind to the resin. iii. A higher concentration of salt solution would be needed to elute protein A from the column than to elute protein B. iv. We would expect to see equally good separation if the scientist had chosen to use a pH of 2
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