4. In the course material you have been introduced to multiple charging for electrospray spectra. Each peak in the spectrum below can be used to provide a measure of the mass of this protein, you can work out the charge associated with each peak, and measures can be averaged to find the overall mass. Use simultaneous equations to find the charge associated with each peak in the spectra below, which will allow you to calculate the average mass of each species. Include an estimate of the error associated with this measure. x10 5 2 02 OD 659.8 714.7 779.5 857.4 952.6 1071.5 1200 1300 1400 +M 01-13min myz

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Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.