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A professor prepares a buffer solution that they need for the purification of protein from human cell lysates. They did mix weak acid and conjugate base and obtained an INITIAL solution, which is characterized by the parameters shown in the images. Unfortunately, they did not pay attention to the instructions and added an additional 5 ml of 1M NaOH to the initial solution. They were then wondering whether they can still use the solution for their purification experiment and what the pH of the resulting FINAL buffer solution would be? Determine the FINAL pH and provide it in the answer box to one decimal place. Note: You may need to round the numbers to get the required answer. 181691.1212 1 M NaOH 14 13 12 11 10 10 9 8 INITIAL pH of solution: 6.9-> Buffer compound pK: 6.3- 6 Final volume of solution: 200 ml 5 Total buffer compound concentration: 150 mM LASSCO Initial concentration weak acid: 0.03 M Initial concentration conjugate base: 0.12 M 3 2 1

A professor prepares a buffer solution that they need for the purification of protein from human cell lysates. They did mix weak acid and conjugate base and obtained an INITIAL solution, which is characterized by the parameters shown in the images. Unfortunately, they did not pay attention to the instructions and added an additional 5 ml of 1M NaOH to the initial solution. They were then wondering whether they can still use the solution for their purification experiment and what the pH of the resulting FINAL buffer solution would be? Determine the FINAL pH and provide it in the answer box to one decimal place. Note: You may need to round the numbers to get the required answer. 181691.1212 1 M NaOH 14 13 12 11 10 10 9 8 INITIAL pH of solution: 6.9-> Buffer compound pK: 6.3- 6 Final volume of solution: 200 ml 5 Total buffer compound concentration: 150 mM LASSCO Initial concentration weak acid: 0.03 M Initial concentration conjugate base: 0.12 M 3 2 1

Principles of Modern Chemistry
Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter15: Acid–base Equilibria
Section: Chapter Questions
Problem 58P
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A professor prepares a buffer solution that they need for the purification of protein from human cell lysates.

They did mix weak acid and conjugate base and obtained the initial solution, which is characterised by these parameters.

Final volume: 200ml
total buffer compound concentration: 150mM
Intitial concentration weak acid: 0.03M
Initial concentration conjugate base: 0.12M
Initial pH 6.9
buffer compound pka 6.3



A professor prepares a buffer solution that they need for the purification of protein from human cell lysates. They did mix weak acid and conjugate base and obtained an INITIAL solution, which is characterized by the parameters shown in the images. Unfortunately, they did not pay attention to the instructions and added an additional 5 ml of 1M NaOH to the initial solution. They were then wondering whether they can still use the solution for their purification experiment and what the pH of the resulting FINAL buffer solution would be? Determine the FINAL pH and provide it in the answer box to one decimal place. Note: You may need to round the numbers to get the required answer. 181691.1212 1 M NaOH 14 13 12 11 10 10 9 8 INITIAL pH of solution: 6.9-> Buffer compound pK: 6.3- 6 Final volume of solution: 200 ml 5 Total buffer compound concentration: 150 mM LASSCO Initial concentration weak acid: 0.03 M Initial concentration conjugate base: 0.12 M 3 2 1
Transcribed Image Text:A professor prepares a buffer solution that they need for the purification of protein from human cell lysates. They did mix weak acid and conjugate base and obtained an INITIAL solution, which is characterized by the parameters shown in the images. Unfortunately, they did not pay attention to the instructions and added an additional 5 ml of 1M NaOH to the initial solution. They were then wondering whether they can still use the solution for their purification experiment and what the pH of the resulting FINAL buffer solution would be? Determine the FINAL pH and provide it in the answer box to one decimal place. Note: You may need to round the numbers to get the required answer. 181691.1212 1 M NaOH 14 13 12 11 10 10 9 8 INITIAL pH of solution: 6.9-> Buffer compound pK: 6.3- 6 Final volume of solution: 200 ml 5 Total buffer compound concentration: 150 mM LASSCO Initial concentration weak acid: 0.03 M Initial concentration conjugate base: 0.12 M 3 2 1
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