A mutant form of polypeptide hormone angiotensin II has the amino acidcomposition (Asp, Arg, Ile, Met, Phe, Pro, Tyr, Val)The following observations are made:• Trypsin yields a dipeptide containing Asp and Arg and a hexapeptide withall the rest.• Cyanogen bromide cleavage yields a dipeptide containing Phe and Proand a hexapeptide containing all the others.• Chymotrypsin cleaves the hormone into two tetrapeptides, of composition (Asp, Arg, Tyr, Val) and (Ile, Met, Phe, Pro)• The dipeptide of composition (Pro, Phe) cannot be cleaved by either chymotrypsin or carboxypeptidase.What is the sequence of angiotensin II?
A mutant form of polypeptide hormone angiotensin II has the amino acid
composition
(Asp, Arg, Ile, Met, Phe, Pro, Tyr, Val)
The following observations are made:
• Trypsin yields a dipeptide containing Asp and Arg and a hexapeptide with
all the rest.
• Cyanogen bromide cleavage yields a dipeptide containing Phe and Pro
and a hexapeptide containing all the others.
• Chymotrypsin cleaves the hormone into two tetrapeptides, of composition
(Asp, Arg, Tyr, Val) and (Ile, Met, Phe, Pro)
• The dipeptide of composition (Pro, Phe) cannot be cleaved by either chymotrypsin or carboxypeptidase.
What is the sequence of angiotensin II?
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