A mixture of four a-[*p]-labeled ribonucleoside triphosphates was added to permeabilized bacterial cells undergoing DNA replication in the presence of an RNA polymerase inhibitor, and incorporation into high-molecular- weight material was followed over time, as shown in the accompanying graph. After 10 minutes of incubation, a 1000-fold excess of unlabeled ribo- nucleoside triphosphates was added, with the results shown in the graph. Excess rNTPs added 5 10 15 Time, minutes (a) Why was the excess of unlabeled rNTPs added? (b) How could you tell that radioactivity is being incorporated as ribonu- cleotides rather than as an alternative such as reduction to deoxyribonu- dleotides, followed by incorporation? (c) What does this experiment tell you about the process of DNA replication? Incorporation of radioac tivity, cpm
A mixture of four a-[*p]-labeled ribonucleoside triphosphates was added to permeabilized bacterial cells undergoing DNA replication in the presence of an RNA polymerase inhibitor, and incorporation into high-molecular- weight material was followed over time, as shown in the accompanying graph. After 10 minutes of incubation, a 1000-fold excess of unlabeled ribo- nucleoside triphosphates was added, with the results shown in the graph. Excess rNTPs added 5 10 15 Time, minutes (a) Why was the excess of unlabeled rNTPs added? (b) How could you tell that radioactivity is being incorporated as ribonu- cleotides rather than as an alternative such as reduction to deoxyribonu- dleotides, followed by incorporation? (c) What does this experiment tell you about the process of DNA replication? Incorporation of radioac tivity, cpm
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
![A mixture of four a-[*p]-labeled ribonucleoside triphosphates was added
to permeabilized bacterial cells undergoing DNA replication in the presence
of an RNA polymerase inhibitor, and incorporation into high-molecular-
weight material was followed over time, as shown in the accompanying
graph. After 10 minutes of incubation, a 1000-fold excess of unlabeled ribo-
nucleoside triphosphates was added, with the results shown in the graph.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F171f0c61-2a07-4cba-9dc0-0006f82fbeb5%2F818ade7e-6392-4c27-a029-a570aa23544c%2Fkpyg8qq_processed.png&w=3840&q=75)
Transcribed Image Text:A mixture of four a-[*p]-labeled ribonucleoside triphosphates was added
to permeabilized bacterial cells undergoing DNA replication in the presence
of an RNA polymerase inhibitor, and incorporation into high-molecular-
weight material was followed over time, as shown in the accompanying
graph. After 10 minutes of incubation, a 1000-fold excess of unlabeled ribo-
nucleoside triphosphates was added, with the results shown in the graph.
![Excess
rNTPs
added
5
10
15
Time, minutes
(a) Why was the excess of unlabeled rNTPs added?
(b) How could you tell that radioactivity is being incorporated as ribonu-
cleotides rather than as an alternative such as reduction to deoxyribonu-
dleotides, followed by incorporation?
(c) What does this experiment tell you about the process of DNA replication?
Incorporation of radioac tivity, cpm](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F171f0c61-2a07-4cba-9dc0-0006f82fbeb5%2F818ade7e-6392-4c27-a029-a570aa23544c%2Flfcd18_processed.png&w=3840&q=75)
Transcribed Image Text:Excess
rNTPs
added
5
10
15
Time, minutes
(a) Why was the excess of unlabeled rNTPs added?
(b) How could you tell that radioactivity is being incorporated as ribonu-
cleotides rather than as an alternative such as reduction to deoxyribonu-
dleotides, followed by incorporation?
(c) What does this experiment tell you about the process of DNA replication?
Incorporation of radioac tivity, cpm
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