A mixture of four a-[*p]-labeled ribonucleoside triphosphates was added to permeabilized bacterial cells undergoing DNA replication in the presence of an RNA polymerase inhibitor, and incorporation into high-molecular- weight material was followed over time, as shown in the accompanying graph. After 10 minutes of incubation, a 1000-fold excess of unlabeled ribo- nucleoside triphosphates was added, with the results shown in the graph. Excess rNTPs added 5 10 15 Time, minutes (a) Why was the excess of unlabeled rNTPs added? (b) How could you tell that radioactivity is being incorporated as ribonu- cleotides rather than as an alternative such as reduction to deoxyribonu- dleotides, followed by incorporation? (c) What does this experiment tell you about the process of DNA replication? Incorporation of radioac tivity, cpm

A mixture of four a-[*p]-labeled ribonucleoside triphosphates was added to permeabilized bacterial cells undergoing DNA replication in the presence of an RNA polymerase inhibitor, and incorporation into high-molecular- weight material was followed over time, as shown in the accompanying graph. After 10 minutes of incubation, a 1000-fold excess of unlabeled ribo- nucleoside triphosphates was added, with the results shown in the graph. Excess rNTPs added 5 10 15 Time, minutes (a) Why was the excess of unlabeled rNTPs added? (b) How could you tell that radioactivity is being incorporated as ribonu- cleotides rather than as an alternative such as reduction to deoxyribonu- dleotides, followed by incorporation? (c) What does this experiment tell you about the process of DNA replication? Incorporation of radioac tivity, cpm

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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