A medium containing 50 g/L of S (substrate) is continuously fed into a completely mixed reaction vessel at a certain liquid flow rate. The working volume of the reactor is maintained at a constant liquid volume of 10 L by overflowing. The following table shows the steady state concentrations of X (biomass or cell), S, and P (product). X (g cells/L) S (g/L) P (g/L) 2.0 30.0 18.0 3.0 20.0 27.0 4.0 10.0 36.0 4.5 5.0 40.5 4.8 2.0 43.2
A medium containing 50 g/L of S (substrate) is continuously fed into a completely mixed reaction vessel at a certain liquid flow rate. The working volume of the reactor is maintained at a constant liquid volume of 10 L by overflowing. The following table shows the steady state concentrations of X (biomass or cell), S, and P (product).
X (g cells/L) S (g/L) P (g/L)
2.0 30.0 18.0
3.0 20.0 27.0
4.0 10.0 36.0
4.5 5.0 40.5
4.8 2.0 43.2
If cell growth follows the Monod equation, no cell death occurs at steady state, and the product formation is growth-associated, determine (1) μmax, (2) Ks, (3) Yx/s, and (4) Yp/s.
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